Products - Microarray Scanners - Laser Scanners - InnoScan 900 Series and MAPIX Software
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1 Products - Microarray Scanners - Laser Scanners - InnoScan 900 Series and MAPIX Software Arrayit offers the world s only next generation microarray scanning technology, with proprietary rotary motion control, dynamic auto-focusing, 2-color fluorescence detection, 0.5 µm scan resolution, 3 minute scan times, 24-substrate slide autoloader option and complete MAPIX software. Product Description Next generation microarray scanning technology. World s only sub 1 µm microarray substrate slide scanner on the market. Proprietary rotary scanning architecture permits the fastest scanning speeds. 3 minutes to scan an entire 25 x 76 mm substrate slide. Scan 24 substrate slides in 100 minutes using the 900AL. Rotary scanning architecture reduces vibrational noise and increases image quality. Much greater reliability and longevity than conventional raster scanners. High resolution acquisition system: scans spot sizes down to 5 µm diameter. Real-time image acquisition for all microarray types including but not limited to in-situ high density glass slide microarrays, spotted microarrays of all kinds, tissue and cell microarrays. Low background noise and high sensitivity system. Uniform scanning across the entire microarray surface regardless of substrate type (glass, membrane and plastic). Not sensitive to shocks, vibrations and slide deformation (portable). Delivered with Mapix software: an easy to use data acquisition and image analysis software including automatic spot recognition and powerful image analysis Software for various OS environments (Linux, Windows ). Ethernet interface allows easy data sharing (scanner can be shared over network by several users). Affordable price, low maintenance. Lightweight, robust structure makes it portable system. Small footprint for laboratory space saving. Only 500 nm resolution microarray scanner available.
2 Specifications Lasers Excitation wavelengths Laser power Gain PMT 0-100% Autofocus Detection Optical resolution 1 µm Thermoelectrically cooled laser diodes with stabilized feedback output 635 nm and 532 nm (900A and 900AL models) 635 nm (900R model) 532 nm (900G model) Custom wavelengths are available. Please contact us with your requirements. Adjustable to two different power settings Real-time and automated Pixel size 1 µm to 40 µm Barcode reader Scanning time Autoloader capacity (optional) Autoloader speed Autoloader throughput Scan area Image format Barcode reader Power supply True confocal and high performances digital laser/pmt system Yes 3.5 min per 22 x 70 mm substrate at 10 µm resolution 24 standard 25 x 76 mm glass substrate slides 5 seconds load/unload per substrate slide 100 min for 24 substrate slides Full substrate slide area (22 x 70 mm) Standard TIFF (16 bit gray scale) All formats VAC Size (L x D x H) 28.3 x 46 x 38.5 cm (models 900A, 900R and 900G) 33 x 60 x 42 cm (model 900AL) Weight Class Sensitivity Dynamic range Uniformity Format Computer interface Image analysis Compatible fluorophores: 17 Kg Class I laser product 0.1 fluor/µm² >10,000-fold <5% CV Open platform glass substrate slides (25 x 76 mm) Ethernet Highly accurate grid positioning using automatic spot recognition provide accurate quantification and 16-bit exportable TIFF data Cy5, Alexa 647, Alexa 660 (635 nm); Cy3, Alexa 546, Alexa 555 (532 nm); and custom wavelengths available Image acquisition and quantification demo available upon request, us at arrayit@arrayit.com.
3 Figure 1. The ArrayIt InnoScan 900 series scanners use dynamic auto focusing, which greatly reduces background noise and increases signal intensities. This proprietary auto-focusing technology allows the system to adjust to temperature changes, mechanical perturbations and other system changes that would otherwise cause fluctuations in signal and background. The result is highly uniform scanning across the entire chip surface and consistent performance scan after scan. Figure 2. Quantitation and analysis software is included with every scanner. Mapix is a microarray image analysis software application that is both intuitive and easy to use, combining imaging and analysis tools for superior performance. Highly efficient algorithms provide rapid and effective feature gridding through automatic spot searching and optimal grid alignment.
4 Figure 3. Automatic spot segmentation ensures high-quality experiment data by selecting relevant pixels and eliminating unwanted ones. Gridding performance and quantification process are adapted to the high resolution of the InnoScan 900 (1 micron) and therefore to the size of images generated. Optimal data visualization and quick learning thanks to its highly interactive commands, with full correlation between displayed data, pictures and plots. Compatible all types of microarrays made byex-situ and in-situ techniques. Reads and analyzes interstitial/staggered/orange packing patterned microarrays made by the NanoPrint, Agilent, Nimblegen and others. Batch processing allows analysis of a large number of microarrays per day to boost productivity (available on Version 3.0.0). Regular software updates are accessible on line to match evolving needs of customers (updates are free for the 1st year). Configurable for full or modular automation of: Scanner control Configuration saving (scan and analysis parameters) Bar code reading Quality control by flagging Compatible with all industry-standard formats and platforms: Support GAL files and recordable transcripts in both text and GPR formats. Images are saved as 16-bit TIFF images with all read parameters and can be copied in JPEG format. Supports image analysis of 16-bit TIFF files from a variety of other scanners. Platform-independent and runs on all operating systems (Windows, 32- and 64-bit Linux and Macintosh).
5 Figure 4. Close up view of the autoloader available on the InnoScan 900AL. Up to 24 microarrays may be loaded per run for fully automated scanning of 24 complete glass substrate slides. Figure 5. Scanned image of a 100 µm diameter microarray spot from an Arrayit H25K Whole Human Genome Microarray manufactured using Arrayit 946 Series contact printing technology. Scan resolution was set at 0.5 µm on the Arrayit InnoScan 900 Microarray Scanner. The Arrayit 900 enables 5 µm feature scanning of microarrays containing up to 50,000,000 (50 million) features per 25 x 76 mm substrate slide.
6 Warranty ArrayIt InnoScan brand products are sold for research purposes. Extreme care and exact attention should be practiced in the use of the materials described herein. All Arrayit products are subject to extensive quality control and are guaranteed to perform as described when used properly. Any problems with these products should be reported to Arrayit immediately. Our liability is limited to the replacement of the product, or a full refund. Any misuse of this product is the full responsibility of the user, and Arrayit makes no warranty or guarantee under such circumstances. Copyright Arrayit Corporation. All rights reserved.
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