GE Healthcare The clear choice for reliable results With Comparative Data New Amersham Hyperfilm
Are you looking for the right film with the clearest results? As a scientist with a passion for research, you deserve to use the best scientific tools to succeed. From chemiluminescent to radioactive detection, it's important to ensure you have the right film for the job a film that offers excellent sensitivity, band clarity and artifact-free, publication-quality images; as well as excellent value for money and the potential for significant cost savings, to make your research budget go further. For over 20 years, Amersham Hyperfilm has remained the film brand researchers in the life sciences have come to trust. When you compare the results generated by Hyperfilm ECL in chemiluminescent and Hyperfilm MP in radioactive detections with Kodak BioMax films...you will clearly see the difference.
the most sensitive chemiluminescence detection film Applications: chemiluminescent detection in Western, Southern and Northern blots Increased sensitivity: greater sensitivity for low concentration protein detection Clearer background: superior contrast and band visibility in the detection of chemiluminescent signals Artifact-free results: minimal occurrence of visual artifacts due to the improved anti-static layer Validated performance: compatibility with ECL, ECL Plus, ECL Advance, CDP-Star and all blue and green chemiluminesence systems compared with Kodak BioMax Light Increased sensitivity and superior contrast Kodak BioMax Light Validated for use with commonly used chemiluminescent reagents A B Fig 1. Western blot detection with Kodak BioMax Light and films. Performance of and Kodak BioMax Light with Western blots of immobilized IgG from mouse protein (5 µg, 2.5 µg, 1.25 µg, 635 ng, 310 ng, 155 ng, 77 ng, and 39 ng). Detected with horse radish peroxidase labeled anti-mouse antibody 1:5000 and Amersham ECL Western Blot detection reagents. C D Publication quality images Kodak BioMax Light Fig 3. A. Western blot of co-immunoprecipitated p53 detected with Amersham ECL detection reagents. Result A kindly supplied by Pamela Robertson, Dept. of Surgery and Molecular Oncology, University of Dundee, UK. B. Western blot of Rabbit IgG detected with Amersham ECL Plus detection reagents. C. Western blot of Rabbit IgG detected with Amersham ECL Advance detection reagents. D. Southern blot of human genomic DNA, detected with n-ras insert probe labeled with Amersham AlkPhos Direct and detected with CDP-Star detection reagents. Fig 2. Southern blot CDP-Star detection with Kodak BioMax Light and Amersham Hyperfilm ECL films. Southern blot of human genomic DNA (1, 0.5, 0.25, 0.12, 0.06, 0.03 µg per lane) detected with n-ras insert probe labeled with Amersham AlkPhos Direct and detected with CDP-Star detection reagents.
the clearest results in radioactive detection Applications: 32 P, 33 P, 125 I Northern, Southern and Western blots 35 S, 33 P, 32 P sequencing Fluorography of 3 H, 14 C, and 35 S High sensitivity: sensitivity down to single-copy gene detection Clearer results: a clearer, colorless background which improves band visibility Flexibility: compatibility for use with all commonly used radioisotopes compared with Kodak BioMax X-Omat AR-5 Superior contrast Kodak BioMax X-Omat AR-5 Excellent sensitivity Kodak BioMax X-Omat AR-5 dpm/band 1991 995 796 398 199 Fig 4. Comparative use of Kodak Biomax X-Omat AR-5 and in Southern blot 32 P detection. Single-copy gene detection using 32 P Southern blot of human genomic DNA isolated from whole blood and digested with HindIII (1, 0.5, 0.25, 0.12, 0.06, 0.03 µg per lane). Detection with n-ras insert probe (1.5 kb) labeled with 32 P- dctp. Autoradiography with at -70ºC with two Amersham Hyperscreen Intensifying Screens for 18 h. 99.5 39.82 19.91 Fig 5. Comparative use of Kodak BioMax X-Omat AR-5 and in dot blots of 32 P (1991, 995, 796, 398, 199, 99.5, 39.82, 19.91 dpm) exposed to each film for 16 h.
Amersham Hyperfilm for high-sensitivity applications High sensitivity: Sensitivity down to single-copy gene detection Speed: Rapid results for when experimental length needs to be kept to a minimum. Whether using chemiluminescent or radioactive detection, high sensitivity is critical for experiments with low target concentrations, low-intensity samples or where experimental length needs to be kept to a minimum. compared with Kodak BioMax MS Increased sensitivity compared with Kodak BioMax MS Excellent performance without a premium price Kodak BioMax MS BioMax MS dpm/band 400 200 100 50 25 12.5 6.25 Fig 9. Western blot analysis with Kodak BioMax MS and films using rabbit IgG (155, 75, 37.5, 18.75, 9.37, 4.7, 2.34, 1.17, 0.58, 0.3 ng) and Amersham ECL Advance detection reagents 5 min exposure. 3.125 1.56 0.78 Fig 10. Comparative use of Kodak BioMax MS and in dot blot experiments using 32 P (400, 200, 100, 50, 25, 12.5, 6.25, 3.125, 1.56, 0.78 dpm) with an exposure time of 24 h.
Amersham Hyperfilm for high resolution applications High resolution: excellent band resolution to visualize fine detail and detect low intensity bands High sensitivity: no compromise on sensitivity Flexibility: compatible for use with all radioisotopes compared with Kodak BioMax MR No compromise on sensitivity Excellent resolution with 33 P BioMax MR dpm/band 1991 Fig 7. Cycle sequencing using Redivue nucleotides ( 33 P) with an overnight exposure to. 995 796 398 199 99.5 39.82 19.91 Fig 6. Application of Kodak BioMax MR and Hyperfilm MP to dot blots of 32 P (1991, 995, 796, 398, 199, 99.5, 39.82, and 19.91 dpm). Exposure time was 16 h for both films. Excellent resolution with 35 S Fig 8. Dideoxy sequence using Redivue Nucleotides ( 35 S) with an overnight exposure to.
Ordering Information Kodak BioMax Light vs. Kodak Kodak Equivalent Hyperfilm code Hyperfilm code Product code Hyperfilm Product 50 sheets 100 sheets BioMax Light-1, 18 x 24 cm, 50 Sheets 8194540 Hyperfilm ECL, 18 x 24 cm 28-9068-36 28-9068-37 BioMax Light-1, 24 x 30 cm, 50 Sheets 1538966 Hyperfilm ECL, 24 x 30 cm 28-9068-40 - BioMax Light-1, 35 x 43 cm, 50 Sheets 8625170 Hyperfilm ECL, 35 x 43 cm 28-9068-41 - BioMax Light-1, 5 x 7 in, 50 Sheets 8689358 Hyperfilm ECL, 5 x 7 in 28-9068-35 - BioMax Light-1, 8 x 10 in, 50 Sheets 1788207 Hyperfilm ECL, 8 x 10 in 28-9068-38 28-9068-39 Kodak BioMax X-Omat AR-5 vs. Kodak Kodak Equivalent Hyperfilm code Hyperfilm code Product code Hyperfilm Product 50 sheets 100 sheets BioMax X-Omat AR-5, 18 x 24 cm, 50 Sheets 8532665 Hyperfilm MP, 18 x 24 cm 28-9068-43 28-9068-44 BioMax X-Omat AR-5, 24 x 30 cm, 50 Sheets 1501451 Hyperfilm MP, 24 x 30 cm 28-9068-47 - BioMax X-Omat AR-5, 35 x 43 cm, 50 Sheets 1651512 Hyperfilm MP, 35 x 43 cm 28-9068-48 - BioMax X-Omat AR-5, 8 x 10 in, 50 Sheets 1651454 Hyperfilm MP 8 x 10 in 28-9068-45 28-9068-46 BioMax X-Omat AR-5, 5 x 7 in, 50 Sheets 1651496 Hyperfilm MP, 5 x 7 in 28-9068-42 - - - Hyperfilm MP, 18 x 24 cm 28-9068-50 - (enveloped) Kodak MS vs. and MP Kodak Kodak Equivalent Hyperfilm code Hyperfilm code Product code Hyperfilm Product 50 sheets 100 sheets BioMax MS-1, 18 x 24 cm, 50 Sheets BioMax MS-1, 24 x 30 cm, 50 Sheets BioMax MS-1, 35 x 43 cm, 50 Sheets BioMax MS-1, 8 x 10 in, 50 Sheets BioMax MS-1, 5 x 7 in, 50 Sheets V8222648 V8326886 V1435726 V8294985 V1111681 Hyperfilm ECL, 18 x 24 cm 28-9068-36 28-9068-37 Hyperfilm MP, 18 x 24 cm 28-9068-43 28-9068-44 Hyperfilm ECL, 24 x 30 cm 28-9068-40 - Hyperfilm MP, 24 x 30 cm 28-9068-47 - Hyperfilm ECL, 35 x 43 cm 28-9068-41 - Hyperfilm MP, 35 x 43 cm 28-9068-48 - Hyperfilm ECL, 8 x 10 in 28-9068-38 28-9068-39 Hyperfilm MP, 8 x 10 in 28-9068-45 28-9068-46 Hyperfilm ECL, 5 x 7 in 28-9068-35 - Hyperfilm MP, 5 x 7 in 28-9068-42 - - - Hyperfilm MP, 18 x 24 cm 28-9068-50 - (enveloped) Kodak MR vs. Kodak Kodak Equivalent Hyperfilm code Hyperfilm code Product code Hyperfilm Product 50 sheets 100 sheets BioMax MR-1, 18 x 24 cm, 50 Sheets 8736936 Hyperfilm MP, 18 x 24 cm 28-9068-43 28-9068-44 BioMax MR-1, 24 x 30 cm, 50 Sheets 8912560 Hyperfilm MP, 24 x 30 cm 28-9068-47 - BioMax MR-1, 35 x 43 cm, 50 Sheets 8715187 Hyperfilm MP, 35 x 43 cm 28-9068-48 - BioMax MR-1, 8 x 10 in, 50 Sheets 8701302 Hyperfilm MP 8 x 10 in 28-9068-45 28-9068-46 BioMax MR-1, 5 x 7 in, 50 Sheets 8941114 Hyperfilm MP, 5 x 7 in 28-9068-42 - - - Hyperfilm MP, 18 x 24 cm 28-9068-50 - (enveloped)
GE, imagination at work and GE monogram are trademarks of General Electric Company. Amersham, Hyperfilm, ECL, ECL Advance, ECL Plus and Redivue are trademarks of GE Healthcare companies. Kodak, BioMax, and X-Omat are trademarks of Eastman Kodak Co. CDP-Star is a trademark of Tropix Inc. 2007 General Electric Company All rights reserved. First published March 2007 All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them. A copy of these terms and conditions is available on request. Contact your local GE Healthcare representative for the most current information. GE Healthcare Bio-Sciences AB Björkgatan 30, 751 84 Uppsala Sweden GE Healthcare Europe, GmbH Munzinger Strasse 5, D-79111 Freiburg Germany GE Healthcare Bio-Sciences Corp. 800 Centennial Avenue, P.O. Box 1327 Piscataway, NJ 08855-1327 USA GE Healthcare Bio-Sciences KK Sanken Bldg., 3-25-1, Hyakunincho Shinjuku-ku, Tokyo 169-0073 Japan Asia Pacific Tel: +85 65 62751830 Fax: +85 65 62751829 Australasia Tel: +61 2 8820 8299 Fax: +61 2 8820 8200 Austria Tel: 01 57606 1613 Fax: 01 57606 1614 Belgium Tel: 0800 73 890 Fax: 02 416 8206 Canada Tel: 1 800 463 5800 Fax: 1 800 567 1008 Central & East Europe Tel: +43 1 972 720 Fax: +43 1 972 722 750 Denmark Tel: +45 70 25 24 50 Fax: +45 45 16 2424 Eire Tel: 1 800 709992 Fax: +44 1494 542010 Finland & Baltics Tel: +358 9 512 3940 Fax: +358 9 512 39439 France Tel: 01 69 35 67 00 Fax: 01 69 41 98 77 Germany Tel: 0800 9080 711 Fax: 0800 9080 712 Greater China Tel: +852 2100 6300 Fax: +852 2100 6338 Italy Tel: 02 26001 320 Fax: 02 26001 399 Japan Tel: 81 3 5331 9336 Fax: 81 3 5331 9370 Korea Tel: 82 2 6201 3700 Fax: 82 2 6201 3803 Latin America Tel: +55 11 3933 7300 Fax: +55 11 3933 7304 Middle East & Africa Tel: +30 210 96 00 687 Fax: +30 210 96 00 693 Netherlands Tel: 0800-82 82 82 1 Fax: 0800-82 82 82 4 Norway Tel: +47 815 65 777 Fax: +47 815 65 666 Portugal Tel: 21 417 7035 Fax: 21 417 3184 Russia, CIS & NIS Tel: +7 495 956 5177 Fax: +7 495 956 5176 Spain Tel: 902 11 72 65 Fax: 935 94 49 65 Sweden Tel: 018 612 1900 Fax: 018 612 1910 Switzerland Tel: 0848 8028 10 Fax: 0848 8028 11 UK Tel: 0800 515 313 Fax: 0800 616 927 USA Tel: +1 800 526 3593 Fax: +1 877 295 8102 GE Healthcare UK Limited Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK www.gelifesciences.com/newhyperfilm 28-9179-26 AA 03/2007