Getting Started Spectra Acquisition Tutorial
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Table of Contents TABLE OF CONTENTS Section 1 - Spectra Acquisition Spectra Acquisition Tutorial...1 Launching the Horizon MBTM FTIR Software...1 Connecting to the Instrument...2 Record a background or a reference spectrum...2 Set the Instrument for Background Spectrum Acquisition...3 Adjusting the Detector Gain Parameter...6 Collect the Background Spectrum...7 Record a sample spectrum...9 Set the instrument for sample spectrum acquisition...9 Collect a sample spectrum...9 Collect a Kinetic Sample Spectrum...10 i
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Section 1 Spectra Acquisition Spectra Acquisition Tutorial Section 1 Spectra Acquisition Spectra Acquisition Tutorial This guide describes the basic steps to acquire a spectrum with the MB3000 Series Spectrometer using the Horizon MB TM FTIR software. Launching the Horizon MB TM FTIR Software STEP 1 Double click the Horizon MB TM FTIR software desktop icon to launch the software. Figure 1-1. Horizon MB TM FTIR desktop Icon. STEP 2 If the User Identification dialog box appears, 1. Enter the required information. 2. Click OK. AA004414-01 Rev. D 1
Connecting to the Instrument Section 1 Spectra Acquisition Connecting to the Instrument STEP 1 In the Instrument Menu, choose Acquisition. Figure 1-2. Instrument menu Wait for the software to detect available instruments. It may take a few seconds. STEP 2 Select your instrument from the Instrument drop-down menu. Figure 1-3. Instrument drop-down menu Record a background or a reference spectrum Remove any sample from the sampling accessory (cell, sample holder etc.). 2 AA004414-01 Rev. D
Section 1 Spectra Acquisition Set the Instrument for Background Spectrum Acquisition Set the Instrument for Background Spectrum Acquisition STEP 1 Click Live. Figure 1-4. Live spectrum button STEP 2 Set the Acquisition and Detector parameters (with the exception of the Resolution field) as shown in Figure 1-5. Figure 1-5. Acquisition and Detector parameters AA004414-01 Rev. D 3
Set the Instrument for Background Spectrum Acquisition Section 1 Spectra Acquisition STEP 3 Set the Resolution to an appropriated value for the type of application or of samples to be analyzed. Figure 1-6. Resolution drop down menu. 4 AA004414-01 Rev. D
Section 1 Spectra Acquisition Set the Instrument for Background Spectrum Acquisition Examine the Health Monitoring status and the Signal Strength value located in the top right corner of the Acquisition window. The Health Monitoring status should not show any error. Typically, the Signal Strength value should be between 25 and 90%. Optimize the detector gain by following the procedure described in section Adjusting the Detector Gain Parameter on page 6. Figure 1-7. Signal Strength AA004414-01 Rev. D 5
Adjusting the Detector Gain Parameter Section 1 Spectra Acquisition Adjusting the Detector Gain Parameter STEP 1 This procedure serves to maximize the signal-to-noise ratio (SNR) by optimizing the signal treatment inside the detector assembly using the gain value. Increase by one level the Detector Gain value Figure 1-8. Adjusting Detector Gain STEP 2 STEP 3 STEP 4 STEP 5 Observe the Health Monitoring tab title and the Signal Strength value. If the Health Monitoring tab title is not highlighted and all Health Monitoring status are OK proceed with Step 3. If this is the case, the Signal Strength (%) should be below 90%. If Health Monitoring is highlighted in RED and the detector status shows an error, proceed with Step 4. Repeat Steps 1 and 2 until the Health Monitoring tab title is highlighted in RED and the detector status shows an error. Signal Strength value displayed should be higher than 90%. Decrease the Detector Gain value by one level. Click Stop when the Detector Gain parameter is set properly. 6 AA004414-01 Rev. D
Section 1 Spectra Acquisition Collect the Background Spectrum Collect the Background Spectrum STEP 1 Set the Acquisition Parameters (with the exception of the Resolution and Scans field) as shown in Figure 1-9. Figure 1-9. Acquisition Parameter for background collection STEP 2 Set Scans parameter to an appropriate value for the type of application or the samples to be analyzed The Sample Information section contains all the saving parameters for the spectrum to be recorded. By default, the spectrum will be named using a nomenclature that includes the spectrum type and count and date/time stamps. By default, the background spectrum will be saved in C:\Documents and Settings\user name\my Documents\Horizon MB\Reference and the sample spectrum will be saved under the data folder in the Measured Data project. These parameters can be configured and edited if needed. STEP 3 Click Reference to start the acquisition of the background spectrum. AA004414-01 Rev. D 7
Collect the Background Spectrum Section 1 Spectra Acquisition Once the spectral acquisition is finished, the message Measurement finished is displayed in the bottom left corner of the Acquisition window. Figure 1-10. Measurement finished message 8 AA004414-01 Rev. D
Section 1 Spectra Acquisition Record a sample spectrum Record a sample spectrum Put a sample in the sampling accessory (cell, sample holder etc.). Set the instrument for sample spectrum acquisition STEP 1 Click Live. Refer to Figure 1-4. STEP 2 Examine the Health Monitoring status and the Signal Strength value located in the top right corner of the Acquisition window. The Health Monitoring status should not show any error. Typically, the Signal Strength value should be between 25 and 90%. Optimize the detector gain by following the procedure described in section Adjusting the Detector Gain Parameter on page 6. STEP 3 Once the Detector Gain parameter is set properly, click Stop. Collect a sample spectrum STEP 1 The Resolution parameter must be set to the same value used to record the background spectrum. Set the Scans parameter. Refer to Step 2 of the Adjusting the Detector Gain Parameter on page 6. AA004414-01 Rev. D 9
Collect a Kinetic Sample Spectrum Section 1 Spectra Acquisition STEP 2 Select the desired Acquisition Mode. Figure 1-11. Acquisition Mode drop down menu STEP 3 STEP 4 The Sample Information section contains all the saving parameters for the spectrum to be recorded. By default, the spectrum will be named using a nomenclature that includes the spectrum type and count and date/time stamps. By default, the background spectrum will be saved in C:\Documents and Settings\user name\my Documents\Horizon MB\Reference and the sample spectrum will be saved under the data folder in the Measured Data project. These parameters can be configured and edited if needed. Click Measure to start the acquisition of the sample spectrum. Once the spectral acquisition is finished, the message Measurement finished is displayed in the bottom left corner of the Acquisition window. Refer to Figure 1-10. Collect a Kinetic Sample Spectrum The Resolution parameter must be set to the same value used to record the background spectrum. STEP 1 Set the Scans parameter. Refer to Step 2 of the Collect the Background Spectrum on page 7. 10 AA004414-01 Rev. D
Section 1 Spectra Acquisition Collect a Kinetic Sample Spectrum STEP 2 STEP 3 STEP 4 Define the number of consecutive spectra to be recorded by setting the value of the Measurement field. If required, define a time delay before the acquisition of the first spectrum by setting the value of the Initial Delay field. If required, define a time interval between consecutive spectra by setting the value of the Interval field. The time interval between consecutive spectra is defined as the time interval between the beginning of the current spectrum acquisition and the beginning of the next spectrum acquisition (i.e. the time interval between consecutive spectra cannot be smaller than the time required to collect one spectrum). STEP 5 Select the desired Acquisition Mode. Refer to Figure 1-11. The Sample Information section contains all the saving parameters for the spectrum to be recorded. By default, the spectrum will be named using a nomenclature that includes the spectrum type and count and date/time stamps. By default, the background spectrum will be saved in C:\Documents and Settings\user name\my Documents\Horizon MB\Reference and the sample spectrum will be saved under the data folder in the Measured Data project. These parameters can be configured and edited if needed. STEP 6 Click Measure to start the acquisition of the sample spectrum acquisition. Once the spectral acquisition is finished, the message Measurement finished is displayed in the bottom left corner of the Acquisition window. Refer to Figure 1-10. AA004414-01 Rev. D 11
Collect a Kinetic Sample Spectrum Section 1 Spectra Acquisition At the end of the acquisition the spectrum is displayed in the main software window, ready to be processed by any Horizon MB TM FTIR tools available according to the purchased software license. Figure 1-12. Recorded spectrum ready to be processed by any Horizon MB TM FTIR tools available 12 AA004414-01 Rev. D
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AA004414-01 Rev. D 4/2011 Copyright 2011 by ABB. All Rights Reserved Registered Trademark of ABB. Trademark of ABB. http://www.abb.com ABB Analytical Measurement 585 Charest Blvd East, suite 300 Québec, Québec G1K 9H4 Canada Phone: 418-877-2944 North America: 800-HELP-365 email: ftirservice@us.abb.com Canada: 800-858-3847 Elsewhere: 418-877-2944 email: ftirsupport@ca.abb.com