Standardized State-Listed Animal Survey Procedures For Use in the Review of SWFRPC Projects James W. Beever III Regional Planner Southwest Florida Regional Planning Council 1926 Victoria Avenue Fort Myers, Fl 33901 (239) 338-2550 ext 224 FAX (239) 338-2560 EMAIL jbeever@swfrpc.org WEBSITE www.swfrpc.com July 17, 2006 First Edition James W. Beever III- Standard State-Listed Species Survey Methods for SWFRPC

1 First Edition The primary purpose of this document is to establish a standardized recommended survey methodology for documented and potential state-listed species in the project vicinity in sufficient detail to allow a meaningful evaluation of the impact of a proposed projects in the Southwest Florida Regional Planning Council (SWFRPC) on those species that have been afforded listed species status by the State of Florida. For each species and species group, this document presents a survey methodology designed to aid in determining the on-site status of the species. The recommended methods range from general, qualitative reviews to specific quantitative procedures. Some species are extremely difficult to detect due to seasonality of occurrence, large individual ranges, cryptic lifestyle and behavior, or low population size. Cursory survey findings that fail to detect the occurrence of a listed species on the project do not automatically imply species absence. Some situations will require a more intensive sampling approach, coupled with an examination of museum records and the current literature, before an on-site status determination can be made. The geographic area covered by this document includes Charlotte, Collier, Glades, Hendry, Lee, and Sarasota Counties. This document does not cover surveys for pelagic marine mammal species that are found in the deeper near shore habitats and that occasionally strand on the shorelines of the coastal counties in the region. In all cases, the investigator (SWFRPC staff or their consultants) should contact the Florida Fish and Wildlife Conservation Commission (FWC) biologists and the staff of the U.S. Fish and Wildlife Service (USFWS), Vero Beach field office for pre-survey assistance in determining the survey information requirements for a given project. In many cases, prior to the survey, investigators are advised to consult the current pertinent scientific literature, including the known geographic range and presence of suitable habitats, and consider the probability of species occurrence within the project area. Some situations may require that the methodologies presented below be modified in order either to achieve the desired level of accuracy or to better reflect actual conditions on a site. Recommended procedure for addressing listed species concerns for the proposed project consists of five (5) steps: STEP 1 1) Review of existing data and literature, and consultation with area biologists and species experts; 2) On-site surveys of specific habitats for potential state-listed species; 3) Avoidance of impacts to confirmed listed species; 4) Minimization of impacts to confirmed listed species; 5) Mitigation of unavoidable impacts to confirmed listed species. This document addresses step 1 and 2 as follows. Dependence upon single external expert sources or a limited range of scientific literature is not a sufficient review of existing data. For example, existing published range maps may not contain complete up-to-date information on the distribution of listed species. Use of all of available data sources increases the accuracy, utility, and veracity of the listed species survey. An accurate map of the habitat types within the project and areas within one mile of the project should be made during the initial project review. The map should follow the Florida Land Use, Cover and Forms Classification System (FLUCFCS) (Florida Department of Transportation 1999). This FLUCFCS mapping should reflect the actual habitat as characterized by vegetation type, not the human land use or zoning of the site. James W. Beever III- Standard State-Listed Species Survey Methods for SWFRPC

2 A list of potential listed species for mapped areas should be generated, based on the habitat types present. This list is available from the HCS staff upon request. The FLUCFCS map and a list of listed species should be provided to HCS for review. Existing data sources may confirm the presence of listed species for the project area. A table should be created that indicates which species have been confirmed and which species potentially occur on the site. Additional surveys may or may not be necessary for those confirmed species, depending on how impacts to these species are addressed. Upon completion of step 1, the project applicant, the FWC, the USFWS, and the SWFRPC will have a complete and agreed-upon FLUCFCS habitat map of the site, a list of potential listed species for the specific project, and an indication of the species that have been confirmed as present on-site by existing data sources. STEP 2 For those species that have been identified to be present or potentially present in the habitats on the project site, a variety of specific site survey techniques exist. The specific sampling methodologies range from simple to complex, depending upon the level of verification required and the species of interest. For example: The presence of the gopher tortoise is easily confirmed by a walking transect of the habitat for visual observation of burrows. In contrast, the presence of a gopher tortoise burrow commensal such as the Florida mouse or gopher frog may require a complex trapping program. Furthermore, while determining gopher tortoise presence is not difficult, but the determination of gopher tortoise population densities and habitat quality for gopher tortoises requires more detailed and complex field effort. For improved organization and to avoid repetition, the following survey methods for listed species are provided by species group and habitat group where practical or, alternatively, by species. In the following discussion, when a FLUCFCS number is indicated in brackets following a habitat, as for example Wetlands (600), this indicates all wetland cover types; when indicated as Coniferous Swamp (620), this indicates all coniferous swamp wetland types; and when indicated as Pine Flatwoods (411), this indicates all pine flatwood types, including xeric, mesic, and hydric pine flatwoods. SEA TURTLE GROUP Atlantic Green Turtle, Atlantic Hawksbill Turtle, Atlantic Loggerhead Turtle, Atlantic Ridley Turtle, Leatherback Turtle These endangered and threatened sea turtles are found typically in association with Tidal Passes, Estuarine Waters (540), Sea Grass Beds (911) and Nesting Beaches (181, 652, 710). The green turtle nests on the east cost, from Volusia to Dade County. Population concentrations of adults occur in Sea Grass systems (911) of the Gulf of Mexico, Florida Bay and the Indian River Lagoon (Ehrhart and Witherington 1992). The hawksbill turtle is associated with hard bottom communities (no existing FLUCFCS code) north to Duval County on the east coast and north to Levy County on the west coast. Diffuse nesting can occur from Cape Canaveral to the Dry Tortugas (Meylan 1992). Ridley turtles occur south to Cape Canaveral on the east coast and along the entire Gulf of Mexico coast (Ogren 1992). One successful nesting is known from Pinellas County. Young forage over Sand and Mud Bottoms (650) of 2

3 First Edition less than 2-m (6.6-feet) depth. Loggerhead turtles nest on beaches west of the Apalachicola River, south of Pasco County and north of Dade County. They utilize a wide range of marine habitats (540, 650, 911) (Dodd 1992). Surveys for sea turtle presence will typically begin with a request for information from the Florida Fish and Wildlife Research Institute (FWRI) in St. Petersburg, consultation with the local turtle watch organization, review with marine herpetologists familiar with the project area, and consultation with local government biologists, (USFWS, FWC, Department of Environmental Protection (DEP), and local jurisdiction). Local commercial fishermen, dive shops, and marine laboratories, such as Mote Marine Laboratory will have information on water body use in their areas. These sources will be likely to have pertinent data on sea turtle use for specific beaches and areas. If specific site surveys are necessary, due to lack of existing documentation, a standard survey method for nesting beaches consists of nightly "turtle watch walks. This consists of a linear transect walks of a beach frontage from one hour after sunset to one hour before dawn during sea turtle nesting season (May through September). Turtles, turtle crawls, and turtles nests confirm presence. More involved survey methods include tagging, egg counts, egg removal for protection, etc. These are not typically necessary for FDOT projects. Surveys of water bodies for sea turtle presence are complex and typically require a fully outfitted trawling vessel capable of operating in the water depths and site conditions of the water body. A review of surveyors capabilities to perform such surveys should be made with the FWRI and permits for accidental take will be needed from USFWS. A basic survey would involve two weeks of timed controlled trawls during the season of presence for the species (typically April through October) in the area. BEACH AND SHOREBIRD GROUP Piping Plover, Southeastern Snowy Plover, Least Tern, Roseate Tern, Black Skimmer, American Oystercatcher The endangered snowy plover requires large open areas of dry Sandy Beaches (181, 652, 710) for breeding and tidal unvegetated Sand Flats (181, 652, 650, 710) for feeding. The piping plover also feeds and roosts in the same habitats. The least tern requires open areas of dry, open areas that are typically Sandy Beaches (181, 652 and 710) for breeding. They will also nest on building rooftops in Commercial (140), Industrial (150), and Institutional (170) areas near feeding water bodies; at Mine Sites (160) particularly on Sand Mines (162); on other sandy Open Land (190, 260, 720), sandy Disturbed Land (741, 742, 743 and 744); on sandy parts of Airports (811), Ports (815), and Facilities Under Construction (819, 829, 839). Least terns forage in a wide variety of water bodies, including Marine, Estuarine and Riverine systems, Borrow Pits, and Lakes (500, 641, 642, 643, 650, 911). The roseate tern requires nests on sandy Beaches (181, 652, 710) and sandy Spoil Areas (743). Roseate terns forage in Marine and Estuarine systems (540, 642, 650, 911). The black skimmer requires dry, open areas that are typically sandy Beaches (181, 652, 710) for breeding. They will also nest on other sandy Open Land (190, 260, 720), sandy Disturbed Land (741, 742, 743, 744), sandy parts of Airports (811), Ports (815), and Facilities Under Construction (819, 829, 839). Black skimmers forage in a wide variety of water bodies including Marine, Estuarine and Riverine systems, Borrow Pits, and Lakes (500, 641, 642, 643, 650, 911). James W. Beever III- Standard State-Listed Species Survey Methods for SWFRPC

4 The American oystercatcher requires broad open areas of sandy Beaches (181, 652, 710) for breeding and a Mudflats (650, 651, 652) and Mollusk Beds (654) for feeding. Generally, large, regularly used nesting areas for the plovers, terns, and oystercatcher are known and documented by the FWC, the Florida Natural Areas Inventory (FNAI) (Johnson and Muller 1992), and the Audubon Society. Use of disturbed sites, buildings, mines, and spoil areas tends to be less well documented. Small groups of shorebirds are less conspicuous and therefore less well documented. Of this group, oystercatcher nesting areas are the least well known. All suitable habitats indicated above should be surveyed for the listed shorebirds for a minimum of five days. The days do not need to be consecutive. Surveys may be performed at any daylight hour, provided weather conditions are sunny, without rain or high winds. Surveys for the snowy plover, least tern, roseate tern, black skimmer, and oystercatcher must occur in the months of April through August. Surveys for piping plover must occur in the months of September through March. For areas less than 10 acres in size, a visual scanning is usually sufficient. Use of a spotting scope is highly recommended. Long, linear sites (such as beaches) and sites that exceed 10 acres in size should be sampled by either spot or pedestrian surveys to attain complete coverage. Use of vehicles on beaches is not recommended. Estimates of individuals by species should be reported for each survey. All observed nesting sites should be mapped and reported by latitude and longitude. Feeding areas need only be mapped. WADING BIRD AND PELICAN GROUP Wood Stork, Florida Sandhill Crane, Whooping Crane, Roseate Spoonbill, Little Blue Heron, Snowy Egret, Tricolored Heron, Reddish Egret, White Ibis, Limpkin, Brown Pelican Generally, rookeries and nesting areas for the wood stork, spoonbills, egrets, and herons are known and documented by the FWC (Runde et al. 1991), the FNAI, and the Audubon Society. Similarly, brown pelican rookeries are typically known and documented. In contrast, cranes and limpkins nest individually and can be verified by ground survey only. Also, new rookeries are found on occasion in freshwater wetlands. Currently all spoonbill nesting sites in Florida is in Mangroves (612) and Cypress (621). The wood stork, little blue heron, tricolored heron, and snowy egret utilize both freshwater and saltwater Forested systems (610, 620, and 630) for nesting. Sandhill cranes typically nest in circular ephemeral Ponds and Prairies systems (640, 641, and 643). Limpkins utilize Riverine Vegetation (510, 550, 560, 644), Freshwater Forested systems (610, 620, 630), and Marshes (640) for nesting. Wading birds forage in a variety of wetland systems, including hydric pine flatwoods, seasonal wetlands, and artificial systems (wet 160, 181, 182, 184, drainage structures in 200s, 254, 500s, 600s, 710, 720, 731, 742, 911). Critical foraging areas can be determined during the woodstork-nesting season during the late wet season when the dry-down hydrology concentrates fish for breeding season foraging. While rookery areas are found in deep wetland Mangrove Islands (612), Cypress Swamps (621) and Forested Wetlands (610 and 620), the principal foraging areas are in depressional wetlands. This critical foraging habitat of 6-10 inches of water, with an abundance of small fishes and other aquatic life, is essential for foraging at the critical February to April breeding season (Ogden 1978d). Wood stork critical foraging habitat is also found in association with Mangrove Fringes and Islands (612), Seagrass Beds (911), Oyster Bars (654), Productive Unvegetated Mudflats (651), and Tributary Stream Marshes (641 and 642). The Florida sandhill crane prefers Wet Prairies (643), Marshy Lake Margins (652), Low-lying Pasture (211 and 212), Open Marsh (641), and Shallow Flooded Open Areas (653) (Williams 1978). Nesting occurs in marshy depressional ponds vegetated in pickerelweed, arrowhead, fire-flag, maidencane, and other herbaceous wetland vegetation in the upland/wetland matrix. Sparsely canopied Hydric Pine Flatwoods (624), Pastures (200s, 261, 741), 4

5 First Edition and Prairies (310, 321) provide suitable foraging habitat for nesting sandhill cranes, near to ponds and marshes utilized as nesting areas. Sparsely canopied Hydric Pine Flatwoods (624), Pastures (200s, 261, 741), and Prairies (310, 321) provide suitable foraging habitat for nesting sandhill cranes, usually in proximity to ponds and marshes utilized as nesting areas. The Florida sandhill crane will also forage across open recreational areas such as Golf Courses (182). Limpkins utilize slow-moving Freshwater Streams and Rivers (510), Marsh (640s), Cypress Heads (621), Hardwood Swamps (610), and Shoreline (652 Fresh) habitats. Diet consists of apple snails, other snails, freshwater mussels, lizards, insects, frogs, worms, and crustaceans. Nesting occurs in a mat of aquatic vegetation (Nesbitt 1978). The nests are very hard to find. Little blue herons utilize a wide variety of Freshwater Habitats (500 and 600) in Florida. Diet consists of crustaceans, insects, small fish, frogs, and lizards. Rodgers (1978) notes that they appear to prefer foraging in freshwater habitats even when nesting in saltwater wetlands. Nesting colonies are located in Mangroves (612); Cypress (621); Willow, Buttonbush, and Red Maple copses (610); and on wetland tree and shrub islands in the center of Ponds and Lakes (520). Breeding occurs during periods of high water. White ibis utilize a wide variety of Freshwater and Saltwater habitats (500 and 600) in Florida. Diet consists of a variety of invertebrates, particularly burrow dwellers, small fish, frogs, and lizards. They appear to prefer to forage in freshwater habitats even when nesting in saltwater wetlands. Nesting colonies are located in Mangroves (612); Cypress (621); Willow, Buttonbush, and Red Maple copses (610); and on tree islands in the center of Ponds and Lakes (520). Roseate spoonbills nest predominately in Mangrove Forests (612) and are now reported to nest in cypress (621) (Church Roberts, pers.com. 2006) and forage wherever concentrations of small fish and crustaceans allow the birds to utilize their unique bills for feeding (Ogden 1978b). Seasonal Wetlands (653) and Hydric Pine Flatwoods (624) provide foraging areas during the March dispersal to interior freshwater wetlands (Allen 1942). Snowy egrets utilize a wide variety of Freshwater and Saltwater habitats (500 and 600) in Florida. Diet consists of crustaceans, insects, and small fish. Nesting can occur in a variety of wetland trees. Ogden (1978c) includes Willow, Wax Myrtle, and Buttonbush (610) as freshwater colony trees. Breeding in Estuarine (612) and Freshwater rookeries (610) occurs during periods of high water in freshwater wetlands. Tricolored herons utilize a wide variety of Freshwater and Saltwater habitats (500 and 600) in Florida. Diet consists of small fish, crustaceans, and insects. Nesting can occur in a variety of wetland trees. Ogden (1978a) includes Willow, Wax Myrtle, Marsh Elder, Pond Apple, and Buttonbush as Freshwater Colony Trees (610). Breeding in Estuarine (612) and Freshwater (610) rookeries occurs during periods of high water in freshwater wetlands. Brown pelicans occur throughout all Marine, Estuarine, and Riverine (500 and 600) habitats. Nesting on the west coast of Florida occurs exclusively in natural Mangrove canopies (612). Some colonies in the Indian River Lagoon use Australian pine on Spoil Islands. Nesting is typically documented on islands. Regular roosting sites are located on both Mangrove Islands and Mangrove Fringes (612). All Wetlands (600s) and Beaches (181, 652 and 710), particularly shoreline and upland transitional zones, should be surveyed for wading birds and brown pelicans for a minimum of five days at dawn and dusk. The days do not need to be consecutive. A typical schedule would include three dawn and two dusk surveys at each wetland area. Since wading birds are opportunistic in their foraging patterns, survey times should always be selected to indicate seasonal use by the species in question. James W. Beever III- Standard State-Listed Species Survey Methods for SWFRPC

6 For herbaceous and non-vegetated wetlands less than 10 acres in size, a visual scanning of the wetland is usually sufficient. Wetlands that are either Forested (610, 620, 630) or exceed 10 acres in size must be sampled by either spot or pedestrian surveys to attain complete coverage. Aerial surveys and airboats may be utilized under a set of agreed-upon criteria, utilizing experienced observers other than from the vehicle operator. Aerial surveys will not locate limpkin nests. The presence of flightless young limpkins and Florida sandhill cranes is considered presumptive of a nest being present. Surveys for the Florida sandhill crane are only valid to subspecies if performed in a period from March through September, when the migratory sandhill cranes are not present; or if flightless young are observed from January through July. If the applicant specifies that any crane observed is a Florida sandhill crane or, given Florida sandhill crane habitat is present, Florida sandhill crane is present, then the seasonal survey restriction can be omitted. Surveys for the nesting Florida sandhill crane are only valid if performed in a period from January through July. If the applicant specifies that Florida sandhill cranes are nesting on-site, then this seasonal survey restriction can be omitted. Estimates of individual wading birds by species should be reported for each survey. All observed nesting and roosting sites should be mapped and reported by latitude and longitude. Foraging areas should be mapped. LARGE RAPTORS GROUP Snail Kite, Bald Eagle Powerful optics, spotting scopes, and binoculars are recommended in surveying for this group of birds. Snail Kite The snail kite feeds almost entirely on apple snails, which it takes from shallow, open freshwater areas. In addition to permanent Freshwater Marshes (641) snail kites will use Ephemeral Ponds (653), open wet Hydric Pine Flatwoods (624), area near deeper Marsh areas (520), and Drainage Canals (510, 520). Specific perches are used as feeding stations, with accumulations of apple snail shells piled beneath them. Perch trees may be located both in wetlands and in uplands. The locations of many snail kite nesting areas in Florida are available from the FWC and USFWS snail kite experts at each regional FWC office (Appendix A). New nests are started each year, however, and new nest locations and new territories are common. In southwest Florida, the snail kite has been documented from eastern and central Collier County (Sykes 1978), and has extended its range westward to nest in Lehigh Acres in eastern Lee County. The following FLUCFCS classes are potential snail kite habitat: Streams and Waterways (510), Lakes (520), Reservoirs (530), Sloughs (560), open Hydric Pine Flatwoods (6241), Freshwater Marshes (641), Sawgrass (6411), Cattail (6412), Spike Rush (6413), Maidencane (6414), Arrowroot (6416), Wet Prairies (643), Aquatic Vegetation (644), Submergent Aquatic Vegetation (645), Intermittent Ponds (653), and Borrow Areas (742). Snail kite use of a site can also be divided into two broad categories based on the structural characteristics of the vegetation. Type I habitat nesting sites consists of type II habitats with areas of shrubs, small trees, or cattails with 5% canopy closure. Type II habitats are open marsh communities with less than 5% canopy closure and with no more than 4 feet of water depth. To define potential snail kite use of a site prior to transect surveys the Type I and Type II habitats should be mapped. The most effective survey technique for snail kites involves the use of airboats, supplemented with observation from the air and from dry land stations. All transect surveys should pass through all potential snail kite habitats present on the site. Use of airboats may be limited to habitats open enough to allow passage of the airboat and sighting of individual birds at a distance from the vessel. The distance between transect surveys should be spaced according to 6

7 First Edition the limits on visibility imposed by vegetation, water depth and terrain. The initial survey should concentrate on locating kites in the air and then focus closer to roosting and nesting areas. Due to constraints on speed required for airboat travel the survey will involve fast travel between stationary points. Some sites may provide road access on roadways, berms, trails through, or near marshes and these access roads can be utilized to supplement airboat surveys. Surveys must be carried out from November through March unless water levels have remained high into late dry season. Conducting surveys early in the breeding season will enhance the ability to sight snail kites due to conspicuous courtship behavior. Surveys should be conducted during the day, from 1 to 2 hours past sunrise to allow thermals to form, on calm, clear days. Direction of travel along transect surveys should be reversed on alternate surveys to eliminate direction of travel bias. Surveys should be conducted 6 to 8 times with 4 to 7 days between each. The same individual(s) should conduct all surveys to eliminate observer bias. Surveyors should observe from both sides of the air boat, looking for snail kites flying, soaring, hovering, and perched on perching areas in trees. When a snail kite is sighted, the observer should have the airboat stop and record the location. Binoculars or spotting scopes should be used to verify identification. The specific habitat category for each snail kite observed should be recorded at the point where the snail kite was first observed. The estimated perpendicular distance from the center of the transect to the snail kite should be measured with a range finder and plotted on the map. An attempt should be made to identify the sex of the snail kite. If the snail kite leaves or moves to a new location while being observed, the flight direction and, if possible, the location to which the snail kite travels should be noted. Behavior and vocalizations should be noted, especially behavior that would indicate courtship or nesting. The surveyor should look for and record signs of snail kite activity (e.g., piles of apple snail shells, white feces stains at perches or roosts). The surveyor should also look for potential nest sites while conducting foot surveys. Potential nest sites include small trees, low shrubs, and thick clumps of sawgrass or cattails. Locations of snail kites, possible snail kite activity, and potential nest sites should be recorded on a map. Upon completion of all transect surveys, surveys for nest sites should begin. Airboat surveys will be needed to locate nests. The surveyor should return to areas where snail kites were repeatedly located; where snail kite pairs were observed; or where snail kites were exhibiting courtship, breeding, or territorial behavior during the transect surveys. If a snail kite is in sight upon arriving in an area, the surveyor should observe the bird for signs of courtship, nesting behavior, and vocalizations, and look for snail kite nest sites in suitable areas. Estimates of the number of individuals should be reported for each survey. Nest site location should be plotted on map and the compass direction and distance from a permanent landmark should be recorded. In addition, all observed nesting and roosting sites should be mapped and reported by latitude and longitude. Feeding areas need only be mapped. Bald Eagle Southern bald eagles usually utilize pines as nest trees, particularly where this community is located near a riverine or lacustrine foraging area. One- and two-year-old immature bald eagles, and some adults, may remain in Florida year-round. Large groups of eagles of up to 20 or more individuals may be spotted soaring on thermals during fall and spring migration. The locations of most bald eagle nesting territories in Florida are available from the HCS Non-Game staff and the Division of Wildlife eagle experts at each regional FWC office. New nests occur each year, however, and new nest locations and new territories unknown to FWC biologists are possible. Pine flatwood habitats near open bodies of water and landfills should be surveyed for nests. In Monroe, Collier, and Dade counties large mangroves may also support eagle nests. On occasion other tall trees, such as cypress and James W. Beever III- Standard State-Listed Species Survey Methods for SWFRPC

8 Australian pines, may support nests. The following FLUCFCS classes are potential bald eagle habitat: Swimming Beaches (181), Open Land (190), Unimproved Pastures (212), Woodland Pastures (213), Coastal Scrub (322) if tall trees are present, Mixed Rangeland (330), Coniferous Forests (410), Pine Flatwoods (411), Longleaf - Xeric Oak (412), Sand Pine Scrub (413), Pine - Mesic Oak (414), Longleaf - Upland Oak (415), Other Pine (419), Oak - Pine - Hickory (423), Tropical Hardwood Hammock (426), Hardwood - Conifer Mixed (434), Australian Pines (437), Coniferous Tree Plantations (441), Streams and Waterways (510), Lakes (520), Reservoirs (530), Bays and Estuaries (540), Major Springs (550), Sloughs (560), Mangrove Swamps (612), Stream and Lake Swamps (Bottomland) (615), Wetland Coniferous Forests (620), Cypress (621), Pond Pine (622), Hydric Pine Flatwoods (624), Wetland Forested Mixed (630), Vegetated Non-Forested Wetlands (640), Freshwater Marshes (641), Sawgrass (6411), Cattail (6412), Spike Rush (6413), Maidencane (6414), Dog fennel and Low Marsh Grasses (6415), Arrowroot (6416), Saltwater Marshes (642), Cordgrass (6421), Needlerush (6422), Wet Prairies (643), Aquatic Vegetation (644), Submergent Aquatic Vegetation (645), Non-Vegetated (650), Tidal Flats (651), Shorelines (652), Intermittent Ponds (653), Oyster Bars (654), Beaches Other Than Swimming Beaches (710), Sand Other Than Beaches (720), Exposed Rock (730), Disturbed Lands (740), Rural Land in Transition Without Positive Indicators of Intended Activity (741), Borrow Areas (742), Spoil Areas (743), Fill Areas (744), Burned Areas (745), and Sea Grass (911). All potential habitat within 3,000 feet of open water; open wetlands, shoreline, and open water zones (retention ponds, reservoirs, etc.); and landfills should be surveyed for nests and foraging. For open habitat patches less than 10 acres in size, a visual scanning of the area is usually sufficient. Areas that are either forested or exceed 10 acres in size should be sampled by either spot, aerial, or pedestrian surveys to attain complete coverage. Bald eagle use of a site can be divided into two broad categories, based on the structural characteristics of the vegetation. Type I habitats are plant communities with potential nest trees (pines, mangroves, cypress). Type II habitats are those utilized as foraging areas (open water). To define potential bald eagle use of a site prior to transect surveys, Type I and Type II habitats should be mapped. Surveys must be carried out from October through May (November to April is preferred) for confirmation of breeding activity. Surveys should be conducted on calm, clear days. The most effective survey technique for bald eagle nests involves the use of aerial surveys of Type I habitat located near Type II habitat, supplemented with on-the-ground site inspections. In order to use aerial methods for a survey, the aircraft should survey all potential bald eagle habitats present. There should be no helicopter or fixed-wing aircraft operation within a 500-foot vertical distance or a 1,000-foot horizontal distance from a known nest. Observers should be separate individuals from the pilot. Two observers with optics should be located to face opposite sides of the aircraft. The observers should be able to communicate with the pilot so as to direct or redirect flight for improved observation. The distance between aerial transect surveys should be spaced according to the limits on visibility imposed by weather conditions, vegetation, and terrain. Air traffic zones and flight path approaches around airports, which do not allow circling or unusual flight patterns, may limit effective use of aerial survey techniques in some areas. Pedestrian surveys should be conducted in those areas unsuitable for or inaccessible by, aerial survey, or may be performed instead of an aerial survey. Field staff should establish parallel line transects, spaced so that all potential habitat areas that qualify as Type I or Type II habitat are surveyed. Transect length and distances between transect surveys will vary with size of the area, topography, and vegetative structure. All transect surveys should be individually labeled on a map of the project site and individually identified by numbering or labeling. Direction of travel along transect surveys should be reversed on alternate surveys to eliminate direction of travel bias. The same individual(s) should conduct all surveys to eliminate observer bias. Surveyors conducting ground and aerial transect surveys should observe both sides of the transect for bald eagles perched on trees, snags, or poles; nests in trees; and for bald eagles flying or soaring. When a bald eagle is sighted, the observation should be recorded on the map. Binoculars and spotting scopes should be used to verify identification. The specific habitat 8

9 First Edition category for each bald eagle observed should be recorded at the point where the bald eagle was first observed. If the bald eagle leaves or moves to a new location while being observed, the flight direction and the location where the bald eagle lands should be noted. Behavior and vocalizations (ground survey only) should be noted, especially behavior that would indicate courtship or nesting. Surveyors should walk transect surveys at a steady pace. The surveyors should not knowingly intrude closer than 750 feet to a nest. Birds may silently flush before being observed as surveyors approach a territory. The surveyor should look for and record signs of bald eagle activity (e.g., prey remains, feathers, debris including droppings accumulated at roosts and nests). The surveyor should also look for potential nest sites while conducting foot surveys. Locations of bald eagles, bald eagle activity, roost sites, and nest sites should be recorded on a map (plot compass direction and distance from a permanent landmark), preferably including latitude and longitude for nest and roost sites. Habitat type and use should be recorded for bald eagle sightings. Binoculars should be used to differentiate adult eagles from immature birds. AQUATIC GROUP West Indian Manatee, American crocodile, American Alligator, Atlantic Sturgeon, Common Snook, Mangrove Rivulus, Detection of individuals within this group can be difficult since these species are aquatic, generally mobile, and are difficult to observe. For this reason, cursory survey efforts that fail to detect any of these species should not be construed to imply species absence. In areas of documented presence, these species will be considered present if suitable habitat is present. West Indian Manatee Principle manatee feeding areas are located in Sea Grass Beds (911), Submerged Aquatic Vegetation (645), Bank Grasses (644), and overhanging Mangroves (612) and in Rivers and Waterways (500s). Sheltered Bays, Coves, and Canals (540) are important resting, feeding and reproductive area. Major travel routes include Passes, Rivers, and Waterways (510). Manatees utilize natural deep channels and dredged channels to access shallow-water feeding and resting areas. Manatees require unobstructed access through these travel waterways to access important wintering areas in Tributary Creeks (510) and Springs (550). The distribution and abundance of manatees is a function of season and water temperature. Surveys for West Indian manatee presence will typically begin with a request for information from the Florida Marine Research Institute (FMRI) in St. Petersburg or the USFWS Sirenia Laboratory in Gainesville (Appendix A), consultation with the local manatee watch organization, review with marine mammalogists familiar with the project area, and consultation with local government biologists (USFWS, FWC, DEP, and local jurisdiction). Local commercial fishermen, local guides, and marine laboratories, such as Mote Marine Laboratory, will have information on water body use in their areas. In many areas, recent studies (Nabor and Patton 1989) of manatees have identified and mapped preferred feeding areas and travel paths within specific water bodies. On October 24, 1989, the Governor and Cabinet approved a number of recommendations from Department of Natural Resources (DNR) (subsequently DEP and now BPS/FWC) designed to improve manatee protection and boating safety. One recommendation dealt with the development of rules to implement speed zones in 14 counties identified as having significant manatee activity. The FWC has requested that each of these "key" counties either select one of the speed zone options or develop their own site specific manatee protection plan. In those counties with proposed or adopted maps, designated zones are presumptive of manatee presence. If specific site surveys are necessary due to lack of existing documentation, an aerial survey and a fully outfitted vessel capable of operating in the water depths and site conditions of the water body would be required. A review of James W. Beever III- Standard State-Listed Species Survey Methods for SWFRPC

10 the surveyors capabilities to perform such surveys should be made with the FMRI. A basic survey would involve two weeks of timed, controlled transect surveys during season of presence for the species. Near shore areas should be surveyed in late fall to early spring in south Florida and in summer in north Florida; offshore areas should be surveyed in summer in south Florida. Surveys of open waters in winter or of north Florida areas in winter are not acceptable. American crocodile The American crocodile is found in marine and estuarine habitats from south Charlotte Harbor, Lee County to southern Palm Beach County in Mangroves (612), High Marsh (642), and Saline Lakes (no FLUCFCS code). Courtship and mating occur in late winter through early spring. Nesting begins in late April to early May on beaches, stream banks, and levees. Hatching is in late July and early August. Current documented breeding occurs from Cape Sable to Turkey Point on the mainland, on North Key Largo, and on some islands in Florida Bay (Moler 1992). Surveys for American crocodile presence will typically begin with a request for information from the FWC Wildlife Research Laboratory in Gainesville, review with crocodile experts familiar with the project area, and consultation with local government biologists (USFWS, FWC, DEP, and local jurisdiction) including where appropriate Everglades National Park, Crocodile Lake National Wildlife Refuge, Rookery Bay National Marine Estuary, and the Southwest Florida Aquatic Preserves. These sources will be likely to have pertinent data on crocodile use for specific water bodies and areas. If specific site surveys are necessary due to lack of existing documentation an aerial survey and a fully outfitted vessel capable of operating in the water depths and site conditions of the water body would be required. A review of the surveyors capabilities to perform such surveys should be made with the USFWS and FWC. A basic survey would involve two weeks of timed, controlled transect surveys during breeding season. Surveys during nonbreeding season will be deemed informational but not sufficient. American alligator American alligators utilize a wide variety of water bodies (500s) and wetlands (600s). During late winter and early spring, as wetland areas dry down, alligators move across uplands between wetlands. Large adult alligators construct "gator hole" ponds in herbaceous Wetlands (640) and depressional Pools (653). These gator holes become centers of wildlife activity in the dry season. Young alligators utilize shallow wetlands, including Hydric Pine Flatwoods (624) and Freshwater Marshes (640), with a water depth of 1 to 2 feet throughout the wet season. As adults grow larger, they no longer frequent shallow wetlands, but utilize areas with longer hydroperiods, such as swamps, ponds, lakes, and deep marshes (Fogarty and Campbell 1978). Specific site surveys may be necessary, due to lack of existing documentation. This typically requires aerial survey or a fully outfitted vessel capable of operating in the water depths and site conditions of the water body. Surveyor expertise should be documented. A basic survey may involve two weeks of time-controlled transect surveys during the breeding season. Fishes Group The mangrove rivulus is found in Indian River and Brevard counties and is distributed continuously from Dade County to Lee County in Mangrove Swamps (612) and high Saltmarsh (642). They are found in stagnant water with high leaf litter, isolated pools, and crab burrows, including land crabs (Taylor and Snelson 1992). They are also found in mosquito impoundment ditches in the Indian River Lagoon system. The life cycle of the snook is linked to healthy Mangrove (612), Sea Grass (911) and Unvegetated Bottom (650) habitats. Early life stages enter Estuarine (510) tributaries and high Marsh (642) habitats for protection, feeding and 10

11 First Edition osmoregulation. Adults forage in vegetated and unvegetated estuarine habitats, sheltering in mangrove prop root habitat during the day. The Atlantic sturgeon has two subspecies in Florida. The Atlantic subspecies is found in St. Johns River, Port Canaveral, Brevard County, Ft. Pierce Inlet, and Hutchinson Island (St. Lucie County). The Gulf subspecies is found in most major Rivers (510) and Estuaries (540) from the panhandle south to Florida Bay. The Suwannee River is a critical spawning area for Gulf sturgeon (Gilbert 1992). It is anadramous. Adults enter fresh water in mid-february with spawning from March through May in the Suwannee River. Gulf sturgeons migrate downstream from October through December. Preferred habitats lack Vegetation (650). Surveys for listed marine fish presence will typically begin with a request for information from experts familiar with the project area, and consultation with local government biologists (USFWS, FWC, DEP, and local jurisdiction) including where appropriate National Wildlife Refuge, National Marine Estuary, and Aquatic Preserves. Local marine laboratories, such as Mote Marine Laboratory and Harbour Branch Laboratory, will have information on water body use in their areas. If specific site surveys are necessary, due to lack of existing documentation, this typically involves a creel or fish trap survey and may involve a fully outfitted vessel capable of operating in the water depths and site conditions of the water body. A review of surveyors capabilities to perform such surveys should be made with the USFWS and FWC. A basic survey would involve two weeks of trapping during clement weather. PINE FLATWOODS CAVITY NESTING GROUP Red-cockaded Woodpecker, Southeastern American Kestrel, Mastiff Bat The red-cockaded woodpecker in Florida utilizes southern slash pine (Pinus elliottii var. densa) flatwoods, often hydric, as nesting and foraging habitat in south Florida (Beever and Dryden 1992), and upland mesic and xeric longleaf pine (Pinus palustris) forest in north, central, and panhandle Florida (Baker 1978, Bradshaw 1990, Crosby 1971, Henry 1989). The territories of red-cockaded woodpeckers in hydric slash pine flatwoods are documented to be larger, on average 356.7 acres (144.4 h), than in northern longleaf pine, which ranges from 172.4 to 233.2 acres (69.8 to 94.4 h) (Nesbitt et al. 1983, Patterson and Robertson 1981). The following FLUCFCS classes are potential red-cockaded woodpecker habitat: Recreational Land (180), Unimproved Pasture (212), Woodland Pasture (213), Specialty Farms (250), Other Open Lands (260), Coniferous Forests (410), Pine Flatwoods (411), Mixed Pine/Cypress (620), Longleaf-Xeric Oak (412), Pine-Mesic Oak (414), Hardwood-Conifer Mixed (434), Hydric Slash Pine Flatwoods (624), Rural Land in Transition Without Positive Indicators of Intended Activity (741), and Burned Areas (745). Red-cockaded woodpeckers are documented to avoid areas of dense midstory; however, site tenacity influences habitat utilization. Foraging and cavity tree surveys should be conducted at least one-half mile from a roadway corridor in potential habitat (typically open pinelands with limited midstory). Proposed rights-of-way should be surveyed intensively. Detection of foraging red-cockaded woodpeckers requires an extensive time commitment. Red-cockaded woodpecker use of a site can be divided into two broad categories based on use by the birds. Pinelands with mature pine trees of known age of 60 years or more, open midstory, and regular burns are potential colony and foraging habitat areas. Pine trees in this habitat will have a 6-inch diameter at breast height (DBH) or greater. Other habitat, such as areas with sparse pine canopy, areas with melaleuca or Brazilian pepper invasion, mixed pine/cypress habitat, cypress heads, open areas between forested habitats (wax myrtle, saw palmetto), and James W. Beever III- Standard State-Listed Species Survey Methods for SWFRPC

12 very young pine habitat, is utilized in south Florida, although this habitat use may not be typical throughout this species' range. Red-cockaded woodpeckers will forage on 2 to 5 year-old pine trees (1.5 to 3-inch DBH) in south Florida. To define potential use of a site by red-cockaded woodpeckers prior to transect surveys all forested habitat meeting the initial criteria should be mapped. In areas where other habitat use, as stated, has been documented, additional habitats should be mapped for survey. The most effective survey technique for red-cockaded woodpeckers involves the use of parallel pedestrian transect surveys. Surveys from vehicles will often miss red-cockaded woodpecker presence. Pedestrian transect surveys should pass through all potential red-cockaded woodpecker habitats present on the site in order to allow sighting of cavities, start holes, and individual birds. The distance between transect surveys should be spaced according to the limits on visibility imposed by vegetation and terrain. Typically, transect surveys spaced 200 feet apart are acceptable, except for areas of dense midstory or exotic plant invasion. Field staff should establish parallel transect lines spaced so that all potential habitat areas are surveyed. Transect length and distances between transect surveys will vary with size of the area, topography, and vegetative structure. All transect surveys should be individually labeled on a map of the project site. Surveys for red-cockaded woodpecker presence may be performed throughout the year, but are most effective if conducted during the breeding season (late April to early June) and in the fall. Surveys for breeding must be performed during the breeding season (late April to early June). Conducting surveys in the breeding season will enhance the ability to locate active cavity trees and nest sites. Surveys for cavity trees should be performed in early morning or late afternoon when cavities are most visible because of sunlight shining on the trunks. Surveys for foraging red-cockaded woodpeckers are more successful in the early morning hours, at least during hot weather. Direction of travel along transect surveys should be reversed on alternate surveys to eliminate direction of travel bias and tree face bias. Fall surveys are more conducive to documenting the full non-nesting territory of a red-cockaded woodpecker clan. Both nesting season and non-nesting season surveys should be conducted for a period of two weeks each, for each project. Surveyors conducting transect surveys should observe all sides of pine trees; look for red-cockaded woodpeckers foraging on trees and in flight. In the early morning, red-cockaded woodpeckers will often forage with other birds, including other woodpeckers, nuthatches, and warblers. The surveyor should listen for red-cockaded woodpecker activity. Tapes of red-cockaded woodpeckers are available from the Audubon and Peterson field guide series and can be used, but not depended on, in surveying for this species. When a red-cockaded woodpecker is sighted, the observer should stop and record the location. Binoculars and spotting scopes should be used to verify identification. The specific habitat category for each red-cockaded woodpecker should be recorded at the point where the redcockaded woodpecker was first observed. Data should be collected at half-hour intervals. If the red-cockaded woodpecker moves to a new location while being observed, the flight direction and location where the red-cockaded woodpecker lands should be noted. Behavior and vocalizations should be noted, especially behavior that would indicate courtship or nesting. Locations of red-cockaded woodpeckers (including vocalizations), cavity trees, and start holes should be recorded on a map (plot compass direction and distance from a permanent landmark). Habitat type and use should be recorded for red-cockaded woodpecker sightings. Tree species, tree height, tree diameter at breast height (DBH), and activity level should be recorded for start holes and cavity trees. Surveys for cavity tree activity should be conducted after initial transect surveys are surveyed across the site. Surveys to determine cavity tree activity should be conducted during the morning hours, from 1 hour prior to sunrise to 3 to 4 hours past, on calm, clear days. Each cavity should have its individual observer. Cavity trees should be categorized as active, inactive, or abandoned based on appearance and survey of clan activities on the site. Surveyors should observe members of the red-cockaded woodpecker clan for signs of courtship and nesting behavior in late April to early June. Nest site location should be plotted on map, with a record of compass direction and distance from a permanent landmark. The same individual(s) should conduct all surveys to eliminate observer 12

13 First Edition bias. It is not possible to determine that a cluster is inactive by the appearance of the trees alone, especially over short survey times. Using tree appearance criteria alone, active colonies have been reported to be inactive. In physically undisturbed colony sites in southwest Florida, red-cockaded woodpeckers may utilize what appear to be completely inactive or abandoned cavities for roost trees. Studies (Doerr, 1988) indicate that 60% of inactive clusters in some North Carolina study sites are typically re-activated within 10 years and that red-cockaded woodpeckers may reoccupy abandoned clusters of trees due to a shortage of trees from natural events or interspecies competition. Doerr states that in active cluster sites "it is essential that there be an apparent surplus of trees to provide secure roosting for each bird, in a given year each clan usually uses 2-12 or more cavity trees, and that in a given year not all cavity trees are likely to be used." Southeastern American kestrel The southeastern American kestrel is a small falcon that utilizes open habitat for foraging and nests in tree cavities, typically abandoned woodpecker holes in dead pine trees. Foraging is performed from tall pine trees, snags, power and telephone poles and wires, and other tall objects. The kestrel feeds on large insects and, occasionally, on small rodents, reptiles, and birds (Wiley 1978). Open pine flatwoods provide for the life history requirements of the kestrel. The observed foraging areas for these birds often extend to adjacent open habitats, such as pasture, both wet and dry prairies, and mowed roadway edges. Survey guidelines for the resident Florida subspecies are found in Stys (1993) and are recounted here. The following FLUCFCS classes may be potential kestrel habitat: Recreational Land (180), Improved Pasture (211), Unimproved Pasture (212), Woodland Pasture (213), Specialty Farms (250), Other Open Lands (260), Herbaceous Rangeland (310), Coniferous Forests (4101), Pine Flatwoods (4111), Longleaf-Xeric Oak (4121), Pine-Mesic Oak (4141), Xeric Oak (4211), Hardwood-Conifer Mixed (4341), Mixed Hardwood (4381), Forest Regeneration Areas (443), Hydric Slash Pine Flatwoods (624), Rural Land in Transition Without Positive Indicators of Intended Activity (741), and Burned Areas (745). Kestrel use of a site can also be divided into two broad categories based on the structural characteristics of the vegetation. Type I habitats are plant communities with less than 10% canopy closure and with at least 60% herbaceous ground cover of less than 25 cm. in height. Type II habitats are open woodland communities with greater than 10% but less than 25% canopy closure and with at least 60% herbaceous ground cover of less than 25 cm. in height. To define potential kestrel use of a site prior to transect surveys, Type I and Type II habitats should be mapped. The most effective survey technique for kestrels involves the use of vehicle transect surveys supplemented with pedestrian transect surveys. Vehicle transect surveys should include all potential kestrel habitats present on the site, and these habitats should be open enough to allow sighting of individual birds at considerable distance from the vehicle. The distance between transect surveys should be spaced according to the limits on visibility imposed by vegetation and terrain. A driving speed of 10 to 25 MPH is recommended, varying in response to terrain, road condition, or visibility. High-traffic roads that do not allow slow driving or frequent stopping cannot be utilized. On sites with limited vehicular access, vehicular surveys must be supplemented with pedestrian surveys. Surveyors conducting road transect surveys should observe both sides of the road, looking for kestrels perched on fencerows, telephone poles and lines, and trees; and for kestrels flying or hovering. When a kestrel is sighted, the observer should stop the vehicle and record the location (both by odometer reading and on the map). Binoculars and spotting scopes should be used to verify identification. The specific habitat category for each kestrel observed should be recorded at the point where the kestrel was first observed. The perpendicular distance from the center of James W. Beever III- Standard State-Listed Species Survey Methods for SWFRPC