Aqualog. Water Quality Measurements Made Easy PARTICLE CHARACTERIZATION ELEMENTAL ANALYSIS FLUORESCENCE

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Aqualog Water Quality Measurements Made Easy ELEMENTAL ANALYSIS FLUORESCENCE GRATINGS & OEM SPECTROMETERS OPTICAL COMPONENTS PARTICLE CHARACTERIZATION RAMAN SPECTROSCOPIC ELLIPSOMETRY SPR IMAGING

Water quality measurements made easy The only simultaneous absorbance and fluorescence system for water quality analysis! The new Aqualog is the only instrument to simultaneously measure both absorbance spectra and fluorescence Excitation-Emission Matrices. EEMs are acquired up to 100 times faster than with other instruments. Dedicated software automates traceable Quinine Sulfate Unit calibration and correction of inner-filter effects and Rayleigh and Raman scattering lines, enabling rapid export to multivariate modeling programs including our partner, Solo, by Eigenvector Research, Inc. Hardware features The only true simultaneous absorbance-fluorescence system available TE-cooled CCD fluorescence emission detector for rapid data-acquisition up to 100 times faster than any other benchtop fluorometer Corrected UV-VIS absorbance detection path for stability and accuracy Double-grating excitation monochromator for superior stray light rejection Matching bandpass for absorbance and fluorescence spectra Automatic sample changer option (2- or 4-position) Compatible with flow cells and titrator Full suite of performance validation tests NIST Fluorescence Standard Reference Materials for spectral calibration and correction (SRMs: 2940, 2941, 2942, 2943) Starna Standard Reference Material for Quinine Sulfate Fluorescence Emission Spectral Correction (RM-QS00) NIST Absorbance Standard Reference Materials for Ultraviolet-Visible Spectrophotometry (SRM 931g) Starna Standard Reference Materials for Ultraviolet-Visible Spectrophotometry (RM-06HLKI) Water Raman signal-to-noise evaluation

Filter wheel for automatic order-sorting 150 W vertically mounted xenon source Fully achromatic coupling optics Aberration-corrected emission spectrograph with TE-cooled CCD for rapid emission measurements Aberration-corrected double-grating excitation monochromator for low stray-light performance Reference detector to correct both absorbance and fluorescence detector signals Absorbance detector for simultaneous excitation and emission data-acquisition Excitation-Emission Matrices and absorption spectra from 200 nm!

Software Features Optimized experiment set-up menus minimize user configuration time Complete NIST-traceable corrected fluorescence spectra automatically generated Spectral and kinetic analysis tools for both absorbance and fluorescence data Methods and batch protocols for automating multiple sample measurement Experimental Menu Absorbance spectra Absorbance kinetics Fluorescence emission spectra Fluorescence emission spectra kinetics Combined fluorescence emission spectra and absorbance kinetics Fluorescence excitation-emission matrices (EEMs) Combined excitation-emission matrices and absorbance spectra Trigger-enabling Sample Queue tool for collection of continuous EEMs plus absorbance spectra, correction, and export for up to 1000 samples without interruption. Compatible with multi-position sample changers, operation of flow-through cells and autosamplers. Automated generation of component identification and quantification tables using Eigenvector's Solo Predictor package! Built-in Tools for EEM Analysis Correction of inner-filter effects Rayleigh-masking of first and second orders Normalization (Quinine Sulfate Units or Raman scattering units) Multivariate analysis, including PARAFAC (parallel factor analysis) Batch export of EEMs 2-Dimensional excitation and emission extraction of spectral profiles from EEMs Multivariate Analyses with Our Partner, Eigenvector Save hours of data processing with the combined power of HORIBA Scientific's new Aqualog and Eigenvector's Solo software! Simply import your fully corrected excitation-emission matrix (EEM) data directly from the HORIBA Scientific Aqualog into Eigenvector s Solo software to rapidly perform PARAFAC and many other multivariate analyses pertinent to colored dissolved organic matter (CDOM). The Aqualog package performs all necessary spectral corrections. Quickly assemble EEMs into convenient DataSet objects to easily manage labels, axis scales, and classes, and include or exclude data from the analysis with a simple click. Solo provides the graphical interfaces to quickly manage and analyze EEM data, create and apply models, and interpret results.

CDOM Aqualog Water Quality Applications Oils and PAHs Measure the full UV to NIR spectrum of water contaminants Chlorophyll from Algae Ideal for quantitative hydrologic studies with tracer dyes, using: Resazurin-resorufin Fluorescein Rhodamine Pyranine Quantum Dots CDOM applications: Membrane fouling (microfiltration, reverse osmosis) Microbial and algal activity Carbon fate and cycling activity

Fluorescence Hardware Specifications Parameter Choice of light source Excitation range Excitation bandpass Excitation monochromator Excitation gratings Excitation wavelength accuracy Specification Standard: 150 W ozone-free vertically mounted xenon arc lamp 230 nm to upper limit of emission detector 5 nm Subtractive double monochromator 1200 gr/mm; 250 nm blaze ±1 nm Extended-UV: 150 W vertically mounted xenon arc lamp 200 nm to upper limit of emission detector Choice of detector UV-Visible Red-extended Emission range 250 620 nm 250 800 nm Emission grating 405 gr/mm; 250 nm blaze 285 gr/mm; 350 nm blaze Hardware pixel-binning 0.41, 0.82, 1.64, 3.28 nm/pixel 0.58, 1.16, 2.32, 3.64 nm/pixel Emission bandpass 5 nm Emission spectrograph Fixed, aberration-corrected 140 mm focal length Emission detector TE-cooled back-illuminated CCD Emission integration time 5 ms minimum CCD gain options Sensitivity Weight 2.25 e /cts in high gain, 4.5 e /cts in medium gain, 9 e /cts in low gain Water-Raman SNR > 20 000:1 (RMS method) (350 nm excitation, 30 s integration) 33 kg (72 lbs) Dimensions L W H (618 435 336 mm); (24 17 13 ) Absorbance Hardware Specifications Parameter www.aqualog.com Scanning range Bandpass Slew speed Optical system Detector Wavelength accuracy Wavelength repeatability Photometric accuracy Photometric stability Photometric repeatability Stray light Specification info.sci@horiba.com 200 800 nm (UV lamp) 230 800 nm (Standard lamp) 5 nm Maximum 500 nm/s Corrected single-beam Si photodiode ±1 nm +/ 0.5 nm ±0.01 AU from 0 to 2 A <0.002 AU per h +/ 0.002 AU (0 to 1 AU) <1% measured with KI standard www.horiba.com/scientific USA: +1 732 494 8660 France: +33 (0)1 69 74 72 00 Germany: +49 (0) 89 4623 17-0 UK: +44 (0)20 8204 8142 Italy: +39 2 5760 3050 Japan: +81 (0)3 6206 4717 China: +86 (0)10 8567 9966 Brazil: +55 (0)11 5545 1514 Other: +33 (0)1 64 54 13 00