FTMS Booster X1 High-performance data acquisition system for FT-ICR MS

FTMS Booster X1 High-performance data acquisition system for FT-ICR MS

What is FTMS Booster? The Spectroswiss FTMS Booster X1 is a high-performance data acquisition and analysis system based on state-of-the-art FPGA (field-programmable gate array) technology. Together with our Peak by Peak software, FTMS Booster X1 is a complete state-of-the-art solution for acquisition of time-domain signals (transients), real-time digital signal processing, trigger decoding, and off-line data visualization and processing. Compatible with any FT-ICR MS, FTMS Booster X1 gives your existing instrument a quantum boost in data quality and processing sophistication, helping you to take on the most complex and challenging applications. 2

Data Acquisition Module Embedded computer Processing and visualization of time-domain signals and mass spectra. High-bandwidth chassis PXI Express backplanes for high-speed data transfer. Low footprint and lightweight. High-performance digitizer / FPGA High sample rate analog-to-digital conversion, in-line digital signal processing and advanced triggering. Differential amplifier Low-noise, high-bandwidth amplification of transients. 3 Data storage buffer High-speed streaming and buffering of both transients and mass spectra.

New Generation Architecture Reduced Noise for Improved Sensitivity, Dynamic Range Full Transient Recording for Improved Resolution, Sensitivity Reduced Phase Distortions for Improved Resolution, Mass Accuracy High-throughput in-line FPGA technologies are the basis of the new architecture in FTMS Booster X1. Compared to traditional systems, the fidelity, accuracy and extent of the acquired time-domain data are substantially improved. This translates directly into improved analytical performance. Signal conditioning ADC FPGA High-performance Data bus Host PC Block diagram of FTMS Booster X1. The analog signal from the FT-ICR passes through our signal conditioning sub-system, before digitization in the analog-to-digital converter (ADC). The digital data stream is continuously processed by a high-performance FPGA chip with proprietary firmware. The high definition but compact digitized transients are transferred to the embedded (host) computer for processing and visualization. 4

Seamless Hardware and Software Integration High-Throughput Data Streaming Compatible with Any FT-ICR MS Open File Formats for Greater Flexibility Operates Parallel to Existing Data Acquisition FTMS Booster X1 is compatible with any contemporary FT-ICR MS instrument and requires no major modification or replacement of existing components. Both on-line and off-line software operate parallel to installed products. Two complementary data sets are simultaneously and synchronously acquired. Both Booster-produced and conventional transients or mass spectra can be processed by Peak by Peak and AutoVectis software tools. Booster data is synchronized with metadata produced by the existing software. Parallel, simultaneous acquisition and processing paths maximize instrument and analysis flexibility. 5

Improved Sensitivity Owing to Reduced Analog and Digital Noise Full Profile Mode Mass Spectra Full Transient Recording FTMS Booster X1 reduces noise levels from 20 % to as much as 300 % compared to standard commercial data acquisition systems. AGC target Signal-to-noise performance of a 10 T LTQ FT-ICR MS (Thermo Scientific) equipped with a 2X NADEL ICR cell for data acquired in parallel with a built-in electronics and FTMS Booster X1. The data were measured with myoglobin in a single scan as a function of target AGC setting. Total signal-to-noise ratio = sum of signal amplitudes divided by six standard deviations of the noise in mass range of 600 1500 m / z (preset R=50,000). Single scan mass spectrum of myoglobin (AGC=3e6, preset R=50,000), acquired on a 10 T LTQ FT-ICR MS (Thermo Scientific) equipped with a 2X NADEL ICR cell with FTMS Booster X1 (red) and with the FT-ICR s builtin electronics (black). Total SNR = sum of signal amplitudes divided by six standard deviations of the noise. The latter is represented with dashed lines. 6

Improved Resolution Owing to Full Transient Recording Conventional FT-ICR MS data acquisition systems typically can measure only part of the available transient. FTMS Booster X1 acquires complete transients, maximizing duty cycle. SNR obviously increases, but resolution benefits even more. Increased length of FTMS Booster transient, %. Notably, ion detection is possible during ion accumulation in an external ion trap, e.g., in LTQ. Broadband mass spectra of ubiquitin acquired with built-in acquisition system (black, FT) and with FTMS Booster X1 (red, FT-B) and processed in magnitude mode FT. Both mass spectra are normalized to the base peak of the former mass spectrum. Dashed lines represent six standard deviations of noise. 10 T LTQ FT-ICR MS (Thermo Scientific) equipped with 2X NADEL ICR cell, preset R=12,500, AGC is off, IT=100 ms. 7

Improved Resolution Owing to Reduced Phase Distortion / Absorption Mode FT Data Compatibility with the AutoVectis Software The accurate, high-definition FTMS Booster X1 transients can be transformed in absorption mode to further increase the resolution using the AutoVectis software. Broadband mass spectra of ubiquitin. Black : built-in electronics and magnitude mode FT. Green : FTMS Booster X1 and off-line absorption mode FT. Both mass spectra are normalized to the base peak of the former mass spectrum. The full transient length (see page 7) was used for calculations with FTMS Booster X1. 10 T LTQ FT-ICR MS (Thermo Scientific) equipped with 2X NADEL ICR cell, preset R=12,500, AGC is off, IT=100 ms. Broadband mass spectra of myoglobin. Black : built-in electronics and magnitude mode FT. Green : FTMS Booster X1 and off-line absorption mode FT. Both mass spectra are normalized to the base peak of the former mass spectrum. The transient lengths were the same in both calculations. 7 T LTQ FT Ultra (Thermo Scientific), preset R=50,000, AGC=4e6. 8

Petroleomics Applications FTMS Booster X1 delivers better sensitivity, speed, accuracy, resolution and dynamic range. Everything needed for the most difficult applications, such as petroleomics. Broadband mass spectra of a crude oil fraction. Black : built-in electronics (.raw, reduced profile mode). Red : FTMS Booster X1. Both mass spectra are normalized to the base peak of the former mass spectrum and represented in magnitude mode FT. 10 T LTQ FT-ICR MS (Thermo Scientific) equipped with 2X NADEL ICR cell, preset R=750,000, AGC is off, IT=100 ms. Number of analyte peaks of crude oil fraction with the abundance higher than six standard deviations of noise for (red) FTMS Booster X1 and (black) built-in electronics (.raw, reduced profile mode) picked in the 1 Da mass windows spreading the mass range of 300-1200 m / z. Data correspond to the mass spectra shown above. 9

High-Precision Fourier Transform Ion Cyclotron Resonance Mass Spectrometry at Cyclotron Frequency The 2X NADEL ICR cell uniquely enables FT-ICR MS at the (true) cyclotron frequency by operating in the quadrupolar ion detection mode. It can be implemented on any FT-ICR MS instrument, including LTQ FT-ICR MS from Thermo Scientific and Solarix FT-ICR MS from Bruker Daltonics. Standard hardware and control software are required. 2X NADEL ICR cell design is based on the open-ended cylindrical cell (Ultra ICR cell from Thermo Scientific). The patented 2X NADEL ICR cell consists of four wide aperture excitation grid-electrodes and four narrow aperture detection electrodes, thus NADEL ICR cell [1]. Ion motion simulations of the 2X NADEL ICR cell revealed that a collective motion of a group of ions with a certain initial energy spread is responsible for generation of a new frequency resonance at the (near) true cyclotron frequency. The electric field influence (trapping and space charge fields) on the measured frequency is substantially diminished in comparison with standard ICR cells measuring reduced cyclotron frequency. [1] Tsybin Y. O., et al., Patent application PCT/IB2014/060709 10

Technical Data Content of delivery High-performance data acquisition system for commercial FT-ICR MS instruments System integration Data acquisition in parallel and synchronously with built-in commercial data acquisition systems Data file formats On-demand file formats of output data, including : HDF5, MGF, mzxml, and imzxml System installation System Trigger wire installation (1 x SMB connector): connection with the instrument s computer ; Signal wire installation (1 or 2 x SMB connectors): connection with the instrument s preamplifier Large data sets (e.g., mass spectra in full profile); High duty cycles (fast measurements, e.g. < 1 ms); Continuous recording of long transients (e.g. > 1 h) Embedded computer Intel Core i7 CPU 2.6 GHz, 8 GB RAM, USB 2.0 and 3.0, Ethernet OS : Windows 7 Professional Data visualization On-line and off-line representation of individual or summed transients, frequency or mass spectra Data storage buffer Analog bandwidth Differential input impedance Hardware inputs Dimensions Weight Power supply Warranty User-defined, e.g., 1-2 TB DC 100 MHz 1 MΩ, 50 Ω 3 x SMB connectors 185 x 300 x 210 mm 8.2 kg 100 230 VAC, 50 60 Hz, Max. 200 W 3 years 11

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