Bioanalytik. detect and identify. Over 25 years experience in Imaging. Imaging Software

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detect and identify Over 25 years experience in Imaging Imaging Software

indigo A short introduction Type User ID and Password to log in

indigo A short introduction After entering a password, you see this page.

indigo A short introduction Five Buttons Acquisition for taking images Analysis Projects to define user rights/sample ids Applications to define the test procedure Contact to download new versions

Application - Mode The most recommended mode for biginner is selecting the Single Image Choose Petri Lumi2 2 1 3

Application - Mode In multiday-mode a sample is measured during a period. All images are under one sample id. 1 2 example 3 J14 J17 J21 J24

Application - Mode In multiday-multi -sample-mode more samples are measured during a period. For each sample an area is defined which is automatically cut out and saved as an 1 2 Image. If 5 mice are imaged in one shot, you get 5 single images. example 3

Application Filters 1 New filter slider Enter 1 2 3 Filter name Central wavelength 4 Half bandwidth 5 Transmission

Application Filters 2 New filter wheel Enter Filter name 2 1 3 Central wavelength Half bandwidth Transmission 4 5

Application Selection Select any operation, move it into selections. 1 Any combination is possible!

Application Selection One click shows parameters in the right box 2 Double click opens parameter check box! 1 3

Application Sample size Sample size means the field of veiw 1 2 3 Sample height means the focus distance from the bottom plate to the target 4

Application View Settings Gives you an overview.

Projects - Settings Projects to define user rights/sample ids

Projects Analyse Settings Presetting for Analysis. If all is set to 0, it is auto-scalling.

Projects User rights Select any person. Any combination is possible!

Projects View Settings 1 Gives you an overview. 2

Acquisition- Select An Existing Project Select a project to start the acquistion

Acquisition- Type Sample ID And Generate Sequence Number The date and project name come out automatically Input the sample ID and press the generate key to generate the sequence generate number

Acquisition- Check Filters and Teach In For fluorescence Imaging Press the Check key to check the Ex. filters and the Em. filters

Acquisition- Check Filters and Teach In For fluorescence Imaging For the project containing the fluorescence imaging, press the Teach in key to record the excitation energy During Teach In process, do not leave targeted objects inside the dark chamber

Acquisition- Quick Check And Acquire Press the Quick Check Key to view the focus and position of targeted objects 1.

Acquisition- Quick Check And Acquire 2. Press the Quick Check Key to view the focus and position of targeted objects

Acquisition- Quick Check And Acquire Press the Acquire key to start the acquisition After Acquisition, the image is saved automatically 1

Acquisition- Quick Check And Acquire 2. Press the Acquire key to start the acquisition After Acquisition, the image is saved automatically

Analyse Define everything Rulers for contrast Rulers for signal intensities

Analyse Define everything Rulers for transparency: One for signal One for photo

Analyse tool bar Analyse Powerful tools Analyse tools facilitate the accurate quantitation and comparision of data

Analyse tool bar Analyse Powerful tools Even more sophisticated 1.

Analyse tool bar Analyse Powerful tools Even more sophisticated 2.

Analyse tool bar Analyse Powerful tools Easily print out the 1. report or export to Excel

Analyse tool bar Analyse Powerful tools Easily print out the 2. report or export to Excel

Analyse ease of analysis Define the image Number of your experiments 1.

Analyse ease of analysis Use rulers for contrast 2. and signal intesities

Analyse ease of analysis Apply for sample-id, of all images of this experiment are treated in the same way. 3.

Analyse ease of analysis 4. Finally go can start a slide show

detect and identify Thanks for Your attention Over 25 years experience in Imaging