OPERATION MANUAL FOR ALLIGATOR TUMBLE STIRRER* VP 710A

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OPERATION MANUAL FOR ALLIGATOR TUMBLE STIRRER* VP 710A *US Patent # 6,176,609 TECHNICAL NOTE 270 Figure 1. Alligator Tumble Stirrer VP 710A. WARNING!!!!! Be advised that the Magnetic Tumble Stirrer has very strong magnetic fields coming from a very strong Neodymium Iron Boron drive magnet. People with pacemakers should not get closer than 24 inches. Remove all magnetic influenced tools and objects from the immediate area to prevent them from being pulled onto the magnet or from striking anyone as the objects are pulled towards the magnet. Keep credit cards, watches and other magnetic sensitive items at least 24 inches from the Magnetic Tumble Stirrer s magnetic fields. Do not operate the Magnetic Tumble Stirrer in the close proximity to thick pieces of aluminum or ferromagnetic material. For more information see TUMBLE STIRRER INSTALLATION section. 9823 Pacific Heights Boulevard, Suite T, San Diego, CA 92121 (858) 455-0643 Fax: (858) 455-0703 email: sales@vp-scientific.com

TUMBLE STIRRER INSTALLATION Caution: Operating the Tumble Stirrer in close proximity (within 25mm) of thick pieces (greater than 12mm) of ferromagnetic or aluminum materials, or both, is not recommended. We recommend that the Magnetic Tumble Stirrer be installed as far away from ferromagnetic material as possible. The closer and larger the magnetic material is to the Magnetic Tumble Stirrer, the greater the torque that is required and the slower the maximum speed will be. If this is a significant drag it could result in undue strain on the motor. This strain could burn out the motor. This is not covered under V&P Scientific, Inc. s warranty for this product (see page 4). Placement on a sturdy bench top or table is also recommended. However, thick aluminum table tops or robot decks, as well as aluminum racks or holders, should be avoided. Although aluminum is not magnetic it will cause a drag on a magnetic field due to eddy currents formed when magnetic flux lines pass through it. A large mass of aluminum will cause a significant drag and result in undue strain on the motor. This strain causes the motor to overheat (>60 o C) which could burn out the motor. This is not covered under V&P Scientific, Inc. s warranty for this product (see page 4). Figure 2. Controller for VP 710A showing power switch and PACESETTER push button keypad. OPERATING THE CONTROLLER The PACESETTER controller is factory-set for operation using the push button keypad. Use the following procedure to start the motor and adjust speed. 1. Turn the AC power ON. The LED display will illuminate and show the current setting for output frequency (Hz). The power light just to the left of the LED display will also illuminate. 2. Press the button labeled RUN/STOP. The display will change to show actual output frequency, which will start at 000 and ramp up to the set frequency. Simultaneously, the motor will start and accelerate. 3. Press the button labeled t to increase the output frequency. The motor will accelerate until the t button is released. Note that the output frequency changes at a progressively faster rate the longer the button is pressed. Press the u button to decrease the output frequency. 4. Press the button labeled RUN/STOP again to stop the motor. 5. If the motor does not start promptly and run smoothly, refer to TROUBLESHOOTING in the Appendix. V&P Scientific, Inc. 2 Technical Note 270

OPERATING THE TUMBLE STIRRER The optimal operating speed of the Tumble Stirrer is dependent upon the particular application to be used and needs to be empirically determined. Factors to consider in determining optimal stir speed are the fragility of the objects being stirred, size, shape, composition of the test tube, vial, bottle or well (polypropylene or polystyrene), depth of the microplate wells, volume and viscosity of the liquid, and the type of stir disc or bar used. In general, stirring microbial cultures works best at low speeds. Stirring to resolubilize extracts, or to stir in deep well microplates, requires high speed. The Tumble Stirrer is well suited for either of these types of applications since it can function at minimum as well as at high RPM. Again the speed of stirring needs to be determined empirically for the application. The Tumble Stirrer Motor is controlled by changing the frequency: the higher the frequency in Hertz (Hz), the faster the speed (RPM). The table below converts the frequency (Hz) seen in the digital read-out of the PACESETTER controller to speed of the Tumble Stirrer in RPM. Speed/Hertz Conversion Table VP 710A RPM Hz 45 7.0 65 8.1 85 9.3 105 10.6 125 11.9 145 13.4 165 14.8 185 16.3 205 17.9 225 19.4 245 20.9 265 22.4 285 24.1 300 25.2 400 33.1 500 41.0 600 49.1 700 57.3 750 67.0 The motor is set at the factory to deliver a top speed of 750 RPM which corresponds to a frequency setting on the control of 67.0Hz. Although it is possible to set the frequency higher, neither V&P Scientific nor Bodine recommends operating at the higher settings for long periods of time or under heavy loads. Also note that at low frequency settings the stirrer may not operate. In this case the frequency must first be set high enough to overcome inertia. V&P Scientific, Inc. 3 Technical Note 270

CARE When not in use, turn the power switch off. Do not place the controller in chambers with temperatures above 40 o C. The Tumble Stirrer motor is an AC Inverter motor and requires no maintenance. The deck of the Tumble Stirrer and the outside of the controller are made of ABS. To clean use a mild detergent followed by a water rinse. Do not immerse Tumble Stirrer or its controller in liquid. For chemical compatibility of ABS please see the following link for more information: http://www.coleparmer.com/techinfo/chemcomp.asp Fuses are easily replaced in the rare event that they blow. The fuse panel is located next to the power cord connection plug on the controller. If technical assistance is required, contact: V&P Scientific, Inc. 9823 Pacific Heights Blvd., Suite T San Diego, CA 92121 Ph: 858-455-0643 Fax: 858-455-0703 sales@vp-scientific.com WARRANTY V&P Scientific, Inc. warrants this product to be free from defects in material and workmanship when used under normal laboratory conditions for one year. This warranty begins from the date of delivery by V&P Scientific. In the event this product fails under normal laboratory conditions within the specified period of time because of a defect in material or workmanship, V&P Scientific will, at its option, repair or replace the product. Damage to the product caused by user negligence is not covered. Please keep the special shipping carton in case the unit needs to be shipped back to V&P Scientific. Please contact V&P Scientific at the above address for return authorization and shipping instructions. This warranty is made in lieu of other warranties expressed or implied including the warranties of merchantability and fitness for a particular purpose. V&P Scientific shall not be liable for loss or damages arising from the use of these products nor for consequential damages of any kind. SPECIFICATIONS AND SAFETY PRECAUTIONS The VP 710 series Alligator Tumble Stirrers are driven by a heavy duty three phase, inverter duty, nonsynchronous, parallel shaft, 1/6 horsepower, induction gearmotor, manufactured by Bodine Electric Company. This motor was selected primarily because of its ruggedness and reliability. It is a PWM type inverter that accepts single-phase AC input voltage and converts it to an adjustable frequency three phase AC output voltage. The control for this motor is a Bodine PACESETTER adjustable speed drive. Since the speed of AC induction motors is proportional to the line frequency, adjusting the output frequency of the inverter enables adjustable speed operation of the motor. At output frequencies below the motor s rating, the PACESETTER series inverter simultaneously adjusts applied voltage to the motor so that it is proportional to the applied frequency. This prevents stator saturation and the resultant overheating. At output frequencies above the motor rating, the applied voltage is held constant, so torque output from the motor is proportionately lower at higher frequencies. If the equipment is used in a manner not specified by V&P SCIENTIFIC, INC., the protection provided by the equipment may be impaired. V&P Scientific, Inc. 4 Technical Note 270

SPECIFICATIONS PACESETTER Specifications Input Voltage: 110 to 120 VAC +10%/-15%, 50/60 Hz +/-5%, Single Phase Maximum Output Voltage: 200 to 240 VAC +10%/-15%, Three Phase Output Frequency: 0 to 120 Hz Ambient Operating Temperature: -10 C to 40 C Humidity: 0 to 95% Noncondensing Vibration: <1G (9.8 m/s 2 Enclosure rating: IP 20 Safety Approval: UL Listed Dimensions: Width X Height X Depth: 2.83 x 5.20 x 4.64 (72mm x 132mm x 118mm) Weight: Model 2701 1.7 lb Additional Tumble Stirrer Specifications Equipment Rating: 230V~, 1.6A, 50/60 Hz Ambient Environment: Indoor use, Altitude up to 2000 m, supply voltage not to exceed 10% of nominal, Installation Category II and Pollution Degree 2. Safety Approval: UL Listed SAFETY PRECAUTIONS The use of motor controls, like that of all utilization of concentrated power, is potentially hazardous. The degree of hazard can be greatly reduced by proper design, selection, installation, and use, but all hazards cannot be completely eliminated. The following safety precautions must be observed during all phases of installation, operation, service, and repair of this motor control product. Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design, manufacture and intended use of the products. V&P Scientific assumes no liability for the customer s failure to comply with safety requirements and practices. Throughout this manual, and the PACESETTER Operators Users Manual (see Appendix), a WARNING will highlight a procedure, which presents potential danger to people, and a CAUTION will highlight a possible danger to equipment. Both types of instructions must always be followed. V&P Scientific, Inc. 5 Technical Note 270

WARNING Do not touch the PCB or components on the PCB right after turning off the power. Wait until the power indicator turns off or at least seven minutes. Do not attempt to wire circuitry while power is on. Do not attempt to examine the components and signals on the PCB with the inverter operating. Do not attempt to disassemble or modify internal circuitry, wiring, or components of the inverter. The grounding terminal of the inverter must be grounded properly with 200V class type III standard. CAUTION Do not attempt a dielectric strength test to internal components of the inverter. They are sensitive semiconductor-devices vulnerable to high voltages. Do not connect the output terminals T1(U), T2(V), and T3(W) to an AC power outlet. The CMOS IC on the primary PCB of the inverter is vulnerable to static electrical charges. Avoid contact with the primary PCB of the inverter. WARNING To avoid personnel injury caused by electrical shock, do not remove the cover of the inverter when the power is ON. CAUTION Do not disconnect motor during operation. Otherwise, inverter over-current breakdown may result. FOOTNOTE The above operating instructions apply to operating the Alligator Tumble Stirrer unit in the Basic mode. For information on connecting the PACESETTER controller to external devices or on changing the programmable functions of the controller, refer to Advanced Installation & Operation in Appendix. APPENDIX Instructions for Installation and Operation of PACESETTER controller. V&P Scientific, Inc. 6 Technical Note 270