OIW-Microscopy. 13 September Connor Douglas PhD

Transcription

1 OIW-Microscopy 13 September 2011 Connor Douglas PhD

2 Optical System High Speed 2 mega-pixel CCD Camera Flow rate 30 litres per minute 10x lens magnification gives an image region of 650 x 494um Minimum object diameter 1 μm System is backlit with high power LED to insure the maximum contrast and providing a long life light system in comparison to halogen Maximum object diameter 494 μm System will auto adjust exposure time in respect to the turbidity of the water

3 What is Video Microscopy A digital camera with a magnification lens is used to capture an image The image is then processed to find objects of interest such as oil droplets, solids and gas in water Raw Image Processed Image

4 Objects in Focus In order to get a accurate measurement of the PPM only those objects that are in focus are measured Thus every object that is found is assess to see if it is in focus A combination of the sharpness at the edge of the object, the objects variation in contrast is used to measure if the particle is out of focus Object Out of Focus

5 Gas Due to the system being backlit, gas in the chamber can be distinguished from oil droplets if the object centre is of a lower contrast than its edges The ratio of this contrast can be adjusted in order to optimise for the detection of Gas Gas Particle

6 Oil or Solids To distinguished between oil droplets and solids the shape is measured The shape factor is a ratio of the area of the object too perimeter around the object Oil Solid

7 Measuring PPM The PPM of image is measured by adding the area of all the oil droplets in the image and dividing it by the size of the image. This is then calibrated against a 4 parameter curve. Low PPM High PPM

8 Percentage Measuring Object Size Measurement of Beads of 10um Number in Sample Min Diameter 5.03um Max Diameter 24.78um Mean Diameter 9.85um Dv um Dv um Dv um Standard Deviation Diameter Size /um

9 Measuring Turbidity The first sign of an upset in producing clean water may be a change in the darkness of the water (turbidity). Microscopy system reports the level of turbidity in a simple traffic colour display in the software If the turbidity is high, colour red in the software then an alarm is sent via a 4-20mA connection

10 Measurements For every object measured and image cycle run the following data is stored Concentration Shape/Focus Size Size PPM Shape factor Dv10 Area Concentration Sharpness Dv50 Area EQPC Turbidity Circularity Dv90 Area PED HS Circularity Diameter Volume Aspect Ratio Feret Diameter Volume EQPC Convexity Diameter EQPC Volume PED Elongation Diameter PED Perimeter Length Width EQPC- value for a circle with the same area as the object PED- value for a circle with the same perimeter as the object

11 OIW Software-Fundamentals System has been designed from the bottom up to be easy to use Has the same feel and flexibility as the current Advanced Sensors analyser User can quickly navigate to the relevant data and charts thanks to the simple panel layout structure Users do not need a high understanding of microscopy to used the system

12 OIW Software-Panels Trending Quick View Image Distribution Cumulative Large Image

13 OIW Software Florescence Reading Panel Microscopy Upper Panel Microscopy Lower Panel Change Panel Type Same panel type can be selected for the lower and upper panels

14 OIW Software Quick View Panel Reports the turbidity of the water in a simple traffic light system. Displays important data in large text for all three object types User Can select to view either Image PPM, Dv10, Dv50 or Dv90

15 OIW Software Distribution Dialog Displays the percentage distribution and/or Cumulative for oil, solids gas. Can view the following properties: Diameter, Area, Volume and Shape Via Right click on the charts a Quick Menu is launch in which the user can change the properties of the dialog

16 OIW Software Image Quick Menu User can Zoom in and out of the image Can view the image with and without the found objects Can select which data is displayed in the table

17 OIW Software Large Image All the features of the Image Panel except for the data table Allows the user to view the image in a large format

18 MiView Software MiView is a client software that allows the user to perform tasks remote of the analyser. The software contains three modes: Live View Allows the user to view data live from the analyser Data View- Data and images can be review in this mode Reprocessing- Images can be reprocessed with different parameters

19 MiView Software-LiveView

20 MiView Software-LiveView LiveView: Displays live data from a remote analyser. Includes the following information: Warning Level System Serial No FLU PPM FLU PPM Todays Average Temperature Turbidity Current Image PPM,Dv10,Dv50,Dv90 for Oil,Gas and Solids Average Values over the a selected time period Oil, Solid and Gas Distribution Image Trending Data Image Display FLU PPM Trending Spectrometer Graph

21 MiView Software-DataView

22 MiView Software-DataView DataView: Review Microscopy data via four controls Image Control User can cycle through images. View both raw and processed images. Display Image in full screen mode. Zoom in and out of an Image. Send an Image to be reprocessed Trending Control View trending data on 20 different properties of Oil, Solids and Gas Select different time periods over which to view the data 2 Distribution Controls View Area, diameter, Volume and Shape distribution data for Oil, Solids and Gas Select different time periods over which to view the data

23 MiView Software-Reprocessing

24 MiView Software-Reprocessing Reprocessing: Single and Batch Reprocess Images of Images User can reprocess the image with the following Properties Sharpness Factor Shape Factor Gas Factor Oil Diameter Min-Max Solid Diameter Min-Max Gas Diameter Min-Max PPM Factor Pixel Calibration Can zoom in and out of the image Can measure the size of objects with a line tool Displays 12 results for Oil, Solids and Gas

25 Side Stream Microscopy System variants Ex400 Ex100M Ex1000M Sample in flow or static Measure Image properties for Oil, Solids and Gas Standard Image PPM Calibration Automatic Image PPM Calibration Able to measure Fluorescence Able to perform Spectra Measurement

26 Comparison with other Microscopy Systems Able to measure Fluorescence on the same system Advanced Sensors Canty Jorin Use long life LED for illumination Maintenance Free Cleaning Calibration 4 Parameter 1 Parameter Unknown Auto Calibration of Image PPM View results at sample point View results remote from analyser Image Resolution 2 mpixels mpixels 0.5 mpixels