Package timeseq. July 17, 2017
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1 Type Package Package timeseq July 17, 2017 Title Detecting Differentially Expressed Genes in Time Course RNA-Seq Data Version Date Author Fan Gao, Xiaoxiao Sun Maintainer Fan Gao Uses a negative binomial mixed-effects (NBME) model to detect nonparallel differential expression(npde) genes and parallel differential expression(pde) genes in the time course RNA-seq data. Depends R (>= 3.0.1) Imports gss, lattice, pheatmap, reshape, grdevices, graphics License GPL (>= 2) Suggests rmarkdown, knitr VignetteBuilder knitr Repository CRAN NeedsCompilation no RoxygenNote Date/Publication :33:24 UTC R topics documented: timeseq_1.0.3-package pabp simulate.dt timeseq timeseq.heatmap timeseq.screeplot timeseq.sort Index 9 1
2 2 pabp timeseq_1.0.3-package Statistical Inference for Time Course RNA-Seq Data using a Negative Binomial Mixed-Effects Model Details In this package, we propose a negative binomial mixed-effects (NBME) model to identify differentially expressed (DE) genes, including nonparallel differentially expressed (NPDE) and parallel differentially expressed (PDE) genes, in time course RNA-seq data. Package: timeseq Type: Package Version: Date: License: GPL-2 GPL-3 Maintainer: Fan Gao <fangaohz@gmail.com> Statistics, pages , 2005a. pabp Example Data in Vignette The data pabp (list) has 5 arguments, including data.count, exon.length, gene.names, group.label, reads. Please refer to timeseq.rd for more information.
3 simulate.dt 3 Format data(pabp) A list with 5 arguments: data(pabp) str(pabp) simulate.dt Example Data in Vignette Format The data simulate.dt (list) has 4 arguments, including data.count, gene.names, group.label, reads. Please refer to timeseq.rd for more information. A list with 5 arguments: str(simulate.dt) timeseq Statistical Inference for Time Course RNA-Seq Data using a Negative Binomial Mixed-Effects Model Accurately identifying differentially expressed (DE) genes from time course RNA-seq data has been of tremendous significance in creating a global picture of cellular function. DE genes from the time course RNA-seq data can be classified into two types, parallel DE genes (PDE) and nonparallel DE (NPDE) genes. The former are often biologically irrelevant, whereas the latter are often biologically interesting. In this package, we propose a negative binomial mixed-effects (NBME) model to identify both PDE and NPDE genes in time course RNA-seq data.
4 4 timeseq timeseq(data.count, group.label, gene.names,exon.length = NULL, exon.level = TRUE) Arguments data.count group.label gene.names exon.length exon.level a n by p matrix of expression values. a vector indicating the experimental conditions of each time point. a vector containing all the gene names. a vector containing the length of exons, only used in exon level data. logical:indicating if this is an exon level dataset. Default is TRUE. Details Nonparallel differential expression(npde) genes and parallel differential expression(pde) genes detection. Value A list with components sorted count NPDE.ratio PDE.ratio genenames table data gene.names exon.length group.label group.length group1.length group2.length exon.level an object returned by NPDE.sort function. It contains sorted Kullback Leibler Ratios(KLRs) for identifying DE genes the number of exons for each gene. the NPDE ratios. the PDE ratios. gene names. gene expression values. a n by p matrix of expression values. a vector including all the gene names. length of exons. a vector indicating the experimental conditions of each time point. the total number of time points. the number of time points of condition one. the number of time points of condition two. logical:indicating if this is an exon level dataset. Default is TRUE.
5 timeseq.heatmap 5 Statistics, , ##Exon level data data(pabp) attach(pabp) model.fit <- timeseq(data.count, group.label, gene.names, exon.length) detach(pabp) ##Gene level data (three replicates) attach(simulate.dt) model.fit <- timeseq(data.count, group.label, gene.names, exon.level=false) timeseq.heatmap Heatmap of the Most Significant NDPE Genes Plots the heatmap for the significant NDPE genes. timeseq.heatmap(timeseq.obj, n) Arguments timeseq.obj n an object returned by timeseq function the number of the most significant NPDE genes. It must be a positive integer.
6 6 timeseq.screeplot Statistics, pages , 2005a. attach(simulate.dt) model.fit <- timeseq(data.count, group.label, gene.names, exon.level = FALSE) timeseq.heatmap(model.fit, n = 10) timeseq.screeplot Scree Plot of Kullback Leibler Distance Ratios Plot the scree plot for all genes in the dataset. timeseq.screeplot(timeseq.obj, type = c("barplot", "lines")) Arguments timeseq.obj type an object returned by timeseq function type of plot: "barplot" for bar plot and "lines" for line graph.
7 timeseq.sort 7 Statistics, pages , 2005a. attach(simulate.dt) model.fit <- timeseq(data.count, group.label, gene.names, exon.level = FALSE) timeseq.screeplot(model.fit, "lines") timeseq.sort Sort NDPE Genes by Kullback Leibler Distance Ratios Sort all genes in the dataset by their Kullback Leibler distance ratios. timeseq.sort(genenames, NPDE.ratio, PDE.ratio, table, count) Arguments genenames NPDE.ratio PDE.ratio table count a vector of gene names a vector of Kullback Leibler distance ratios for NPDE genes a vector of Kullback Leibler distance ratios for PDE genes gene expression values. the number of exons for each gene. Value A list with components NPDE_list PDE_list table1 table2 dataframe of NPDE genes sorted by KLRs dataframe of PDE genes sorted by KLRs gene expression values for each gene, corresponding to NPDE_list gene expression values for each gene, corresponding to PDE_list
8 8 timeseq.sort Statistics, pages , 2005a.
9 Index Topic datasets pabp, 2 simulate.dt, 3 Topic package timeseq_1.0.3-package, 2 pabp, 2 simulate.dt, 3 timeseq, 3 timeseq.heatmap, 5 timeseq.screeplot, 6 timeseq.sort, 7 timeseq_1.0.3 (timeseq_1.0.3-package), 2 timeseq_1.0.3-package, 2 9
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