Euro- BioImaging. 4 th Stakeholder Mee7ng January 21-22, ACV Vienna. Jan Ellenberg and Stefan Schönberg Euro- BioImaging Scien9fic Coordinators

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2 Euro- BioImaging 4 th Stakeholder Mee7ng January 21-22, ACV Vienna Jan Ellenberg and Stefan Schönberg Euro- BioImaging Scien9fic Coordinators

3 Participate in the Revolution in Bioimaging Technologies

4 Participate in the Revolution in Bioimaging Technologies

5 Strong Pan-European Support 250 Euro- BioImaging Partners from 28 countries 19 na9onal imaging communi9es formed in Na9onal investment made or planned, EuBI on roadmap in 12 countries 39 Legal Partners, 250 Associated Partners from 28 Countries, > 270 Letters of Intent, > 1400 Stakeholders

6 Key added values of a European RI Why should European countries invest into and run a European distributed research infrastructure for biological and medical imaging? No single European country can run cubng- edge facili9es for all exis9ng and new imaging technologies: 1. By coordina9ng and integra9ng its efforts, Europe can achieve and maintain leadership in imaging technologies in the global research landscape. 2. A pan- European commitment to imaging infrastructure will provide long- term sustainability for research infrastructure in imaging technologies. 3. Na9onal scien9sts will gain open access to all relevant imaging technologies they need in partner countries delivered by the best experts in the field.

7 National Imaging Initiatives in 19 countries

8 National Imaging Initiatives in 19 countries

9 National Imaging Infrastructure with Open Access for national users e.g. Examples of exis9ng and planned na7onal research infrastructures...

10 Nodes of European significance. AMrac9ve and open for transna9onal access. National Contribution to European research infrastructure

11 Distributed research infrastructure model for Euro-BioImaging Mul9modal Technology Nodes Single Technology Flagship Nodes Euro- BioImaging Hub

12 Operational Model TECHNOLOGY NODES WITH OPEN USER ACCESS Euro- BioImaging HUB (Responsibili9es: coordina9on & support, access, data, training; Executes board decisions e.g. evalua9on, upgrade decommissioning; external representa9on) SINGLE TECHNOLOGY FLAGSHIPS offer an innova9ve technology at European leading level MULTIMODAL TECHNOLOGY NODES provide excellence by integra9on of mul9ple imaging technologies at one site EURO- BIOIMAGING INFRASTRUCTURE Na9onal imaging infrastructure e.g. France- BioImaging, GEBI, > 19 COORDINATED NATIONAL IMAGING INITIATIVES

13 European life scien9sts as users Imaging Infrastructure with physical open user access HUB Web- access portal NODES USER TRAINING MULTIMODAL TECHNOLOGY NODE MULTIMODAL TECHNOLOGY NODE FLAGSHIP NODE FLAGSHIP NODE FLAGSHIP NODE FLAGSHIP NODE STAFF TRAINING Data storage and analysis infrastructure User goes home with results for publica9on

14 Testing the Infrastructure Model: Proof-of-Concept Studies

15 Proof-of-Concept Studies Major results Euro-BioImaging received 228 user applications from 23 European countries, as well as USA, Australia, and Singapore. More than 110 users visited 51 imaging facilities in 14 European countries from January to July In total, more than 60% of all users applied for transnational access, 89% left their home town for getting access.

16 Imaging technologies tested for open and transna7onal user access PCS Results Number of visited facili9es Number of user access based on PCS survey WP6 Mul9- modal integrated ALM 38 2 WP7- CLEM 3 4 WP7- Func9onal Imaging 13 2 WP7- High- throughput 5 4 WP7- Super Resolu9on 28 2 WP8- Mesoscopic Imaging 2 WP8- Probes ALM 0 3 WP8- Mul9- Modal Molecular Imaging 7 3 WP8- Probes MI 6 3 WP9- HF- MRI 5 1 WP9- PCI 2 WP10- Image guided Therapy WP10- Popula9on 0 0

17 Imaging technologies tested for open and transna7onal user access PCS Results Number of visited facili9es Number of user access based on PCS survey WP6 Mul9- modal integrated ALM 38 2 WP7- CLEM 3 4 WP7- Func9onal Imaging 13 2 WP7- High- throughput 5 4 WP7- Super Resolu9on 28 2 WP8- Mesoscopic Imaging 2 WP8- Probes ALM 0 3 WP8- Mul9- Modal Molecular Imaging 7 3 WP8- Probes MI 6 3 WP9- HF- MRI 5 1 WP9- PCI 2 WP10- Image guided Therapy WP10- Popula9on 0 0

18 Changing the European Infrastructure Landscape

19 International relations: Australia Collaboration Framework Agreement between Euro-BioImaging and AMMRF Jan Ellenberg (Scientific Coordinator, Euro-BioImaging) and Simon Ringer (Executive Director, AMMRF), after signing the collaboration framework agreement. EMBL Heidelberg, Feb 1 st, 2012

20 International relations: India Collaboration Framework Agreement between Euro-BioImaging and India BioImaging in preparation INDIA BioImaging Jan Ellenberg and Antje Keppler (Euro- BioImaging) at the inauguration of INDIA BioImaging. NCBS Bangalore, Sep 21 st, 2012

21 Biological Imaging PCS study (1 of 89): From cell biology to cancer therapy: a high-throughput microscopy approach for the study of Aurora-A inhibition in human cells Italia Anna Asteri7, CNR - Ins9tute of Molecular Biology and Pathology, Rome, Italy. Visited the Advanced Light Microscopy Facility of the EMBL (Heidelberg) This experience has given novel stimuli to my studies. Furthermore, I have set the bases for future experiments developed in collaboration with the Advanced Light Microscopy Facility. I thank the Euro-BioImaging initiative for having given me this opportunity. (I.A. Asteriti) Used technology High throughput microscopy U2OS cells pre- synchronised at the G1/S transi9on (snapshot from a movie). Results A large number of data, assaying within the same experiment several doses of the Aurora- A inhibitor and prolonging the observa9on over long 9mes, were acquired. The obtained results will be likely to contribute to the understanding of the mito9c roles of Aurora- A, as well as to a future ra9onalisa9on of the use of Aurora- A inhibitors in cancer therapy.

22 Molecular imaging PCS study (1 of 13): Stereochemistry of amminoacid spacers determines the pharmacokinetics of 111In-DOTA-minigastrin analogs for targeting of the gastrin receptor Petra Kolenc Peitl, University Medical Centre Ljubljana, Slovenia Visited the Radboud University Nijmegen Medical Centre I found really inspiring that I was never just an observer but I was an ac9ve actor from the beginning to the end, having the possibility to work with a small animal- dedicated SPECT/CT machine. There was always technical support available from nice and very well skilled people. This concept of work makes the interpreta9on of the final results much easier since you are aware of the cri9cal points in each individual step that leads to the final result. Comparison of kidney reten9on of two In- 111 labeled minigastrin analogues. Used technology SPECT/CT Results The study allowed the quantification of radionucleotide-labeled peptides in the kidneys providing new insights on how to improve kidney protection in peptide receptor radionuclide therapy in the future.

23 Medical Imaging PCS study (1 of 7): Information integration, transcallosal communication, and inter-individual variability during the processing of voiced and unvoiced consonants in speech signals Karsten Specht, University of Bergen, Norway Visited the Leibniz- Ins7tute for Neurobiology, Magdeburg, Germany The en9re process around the Euro- BioImaging PCS studies was very smooth and s9mula9ng. It was a unique opportunity to conduct a study that could complement my earlier studies. (K. Specht) Used technology: 7T MR scanner Results The results are indica9ng a differen9al func9onal asymmetry of the different phone9c aspects, i.e. the lej and the right auditory system responds differently to them. This gives further evidence to current models on the func9onal asymmetry of speech percep9on and helps further iden9fying the informa9on flow in the brain.

24 Proof-of-Concept Studies Major results Imaging facility perspective The staff efforts for user management, sample-preparation and hands-on user training were much higher in most cases than anticipated. For providing external access in the future, additional staff is needed. The average duration of the user visit lasted 2 weeks. 97% of the participating facilities would offer open access in the future after capacity upgrade.

25 Proof-of-Concept Studies Major results User perspective All users rate the Euro-BioImaging PCS access policy (central online application, communication, transparency, time until access) to be very good to excellent. >70% of users rate the validity of the obtained results to be very good to excellent for publication. >90% state that the benefit of the PCS was worth the effort of traveling. 99% of users would make use of Euro-BioImaging open access facilities in the future.

26 The Euro-BioImaging infrastructure model is requested by users and works 97% of the facilities would offer open access after capacity upgrade. 99% of the users would use of Euro-BioImaging open access facilities. Over 60% transnational access, 89% of users left their home town.

27 From Planning to Construction Survey PCS Natl. Communi7es Technical WPs Strategic WPs Infrastructure model for each imaging technology Criteria for nodes Finance Plan Governance & Legal Structure Negotiation with Member States = EuBI Partners Open Call for EuBI Nodes Business plan construction operation Euro-BioImaging Infrastructure

28 1 st Open Call for Euro- BioImaging Nodes Expressions of Interest

29 How to become a EuBI Node

30 1 st Open Call Euro-BioImaging Nodes Expression of Interest The 1 st Open Call runs from 25 January 30 April 2013 Focuses on nodes that provide physical open access to imaging technologies that have demonstrated feasability by e.g. PCS Imaging technologies that were successfully evaluated in PCS: Multi-modal Advanced Light Microscopy Correlative Light-Electron Microscopy Functional Imaging Super Resolution Microscopy High-throughput Microscopy Mesoscopic Imaging Multi-Modal Molecular Imaging Probes-Molecular Imaging Phase contrast Imaging High-field MRI MRI-PET (evaluated by alternative proof-of-feasibility)

31 Eligibility Applicant demonstrates the user need for new capacity (LeMers of Intent from 50% of external users but max. 30 lemers) Applicant guarantees to provide open access for Euro- BioImaging users to at least 50 % of new capacity (comprises instrument 9me, staff capacity, storage capacity, ) Applicant demonstrates exis9ng communica7on with na7onal/regional funders for infrastructure capacity upgrade The applicant demonstrates that the single- sited or mul9- sited Node will cons9tute a legal en7ty, which in its en9rety will become part of Euro- BioImaging and as such a legal contractual partner of Euro- BioImaging Applicants from ESFRI Countries are eligible.

32 General Review General Review Criteria The following General Review Criteria should be addressed in each Expression of Interest: Scientific and technical excellence of the infrastructure Node Quality and scientific field of the academic environment Geographic coverage Maintenance and update European and national significance Access and service package Use and quality assurance User training If applicable, evidence of funding commitment by national funders

33 Common Technology Review Criteria Technology Specific Review

34 Technology Specific Review Specific Technology Review Criteria Example: High-throughput Microscopy Large- scale reagent libraries for cell or organism perturba9ons (e.g. sirna, cdna or chemical libraries) Support in assay development and adapta9on of low throughput laboratory assays to automa9on Support in high- throughput sample prepara9on (e.g. cell seeding devices, liquid handling, robots) High- throughput image acquisi9on technology and live cell imaging Support in large- scale data management and handling Tools and support for automated image analysis of large data sets Tools and support for data mining and evalua9on tools Large- scale compu9ng ( cluster ) to enable rapid analysis of large data sets

35 Summary Open call for Nodes 1st Open Call: 25 Jan 30 Apr st Call focuses on technologies that will provide physical open access to imaging technologies Eligible applicants will be reviewed by general and technology specific review criteria Addi9onal imaging technologies will be added in future open calls ajer passing the Euro- BioImaging technology iden9fica9on process: 1. Demonstrate user need for (new) imaging technology 2. Develop research infrastructure model for providing open access to this imaging technology 3. Demonstra9on of feasibility in PCS

36 To Dos for Node applicants 1. Contact your national imaging community. 2. Talk to your national funders. 3. Submit your Expression of Interest online until April 30 th. Forms and templates will be available on the Euro-BioImaging website on January 25 th, 2013.

37 Webportal Biological Imaging Jan Ellenberg (Scientific Coordinator) Antje Keppler (Project Manager) Medical Imaging Stefan Schönberg (Scientific Coordinator) Pamela Zolda (Project Manager)

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