Microscope & Measuring
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1 Name: ate: 1. microscope is supplied with 10 and 15 eyepieces, and with 10 and 44 objectives. What is the maximum magnification that can be obtained from this microscope? student viewing a specimen under low power of a compound light microscope switched to high power and noticed that the field of view darkened considerably. Which microscope part should the student adjust to brighten the field of view?. diaphragm. coarse adjustment. find adjustment. eyepiece 2. To view cells under the high power of a compound microscope, a student places a slide of the cells on the stage and moves the stage clips over to secure the slide. She then moves the high-power objective into place and focuses on the slide with the coarse adjustment. Two steps in this procedure are incorrect. For this procedure to be correct, she should have focused under. low power using coarse and fine adjustments and then under high power using only the fine adjustment. high power first, then low power using only the fine adjustment. low power using the coarse and fine adjustments and then under high power using coarse and fine adjustments 4. The low-power diameter of the field of a microscope is determined to be 1.5 millimeters. slide of onion skin cells is put on the stage, and exactly 3 cells can be counted lengthwise across the diameter of the field. The average length of each cell is. 100 µ m. 500 µ m. 750 µ m. 1,500 µ m. low power using the fine adjustment and then under high power using only the fine adjustment page 1
2 5. When a student uses a compound microscope and switches from low power to high power, the diameter of the field of view will 8. The total magnification of an image formed by a compound light microscope is a result of the combined magnification of the. decrease. increase. remain the same. eyepiece and diaphragm. objective and mirror. eyepiece and objective. low-power objective and high-power objective 6. If the average length of each cell is 100 µ m, the diameter of the field of vision represented in the diagram is approximately. 50 µ m. 100 µ m. 400 µ m µ m 9. transparent metric ruler is placed on the stage of a microscope and observed under low The diameter of the field of vision was found to be 2 millimeters. How many micrometers is the diameter? ,000. 2, paramecium on a slide can usually be found more easily by using the low-power objective rather than the high-power objective because with low power 10. Which part of a microscope should be used with the low-power objective, but not with the high-power objective?. the field is not as bright. the organism can be seen in greater detail. smaller organisms can be seen. a larger part of the slide can be seen. coarse adjustment. fine adjustment. diaphragm. ocular page 2
3 11. ccording to the diagram shown, the diameter of the black circle shown in the field of view of the microscope is closest to. 200 µ m 13. The diagram represents two cells next to a metric measuring device under the low-power objective of a compound light microscope. What is the approximate length of a nucleus of one of these cells?. 600 µ m. 800 µ m. 1,400 µ m 12. What is the lowest possible magnification that can be obtained using the microscope shown?. 100µ m. 500µ m. 1000µ m. 1500µ m page 3
4 14. student studied the upper layer of cells of a tissue sample under the high-power objective of the compound light microscope as shown. Which part of the microscope should the student adjust to observe the cells in the lower layer of the sample? 15. student used a light microscope to observe a cell under low fter the student switched to high power and attempted to focus, the cell was no longer visible. What was most likely the cause of the disappearance of the cell?. The diaphragm was open while the student observed the cell under low. The distance between the specimen and the objective lens decreased after the student switched to high. The student focused the eyepiece before observing the cell under high. The cell was not in the center of the field of view when the student observed it under low.... page 4
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