SOP: EDAX Eagle III Microspot XRF

Size: px
Start display at page:

Download "SOP: EDAX Eagle III Microspot XRF"

Transcription

1 SOP: EDAX Eagle III Microspot XRF Page 1 of 6 SOP: EDAX Eagle III Microspot XRF 1. Scope 1.1 This document describes the standard operating procedure (SOP) for the EDAX Eagle III Microspot XRF. This X-ray Fluorescence (XRF) tool has the capability to achieve both fast and accurate elemental analysis of specific spots on even bulk samples. Although it is a closed cabinet system, because it uses an x-ray source, using this machine independently requires completing an x-ray safety training 2. Table of Contents 1. Scope Table of Contents Reference Documents Referenced within this Document Safety Setup Procedures Hardware Handles Loading a Sample Lights and controls Viewing the Sample Set up Collecting a Spectrum Getting Counts Characterizing a Spectrum Determining Elements Quantifying Results Shutting Down Saving Process Summary Revision History Reference Documents 3.1 Referenced within this Document None 4. Safety 4.1 Follow all Nanofab safety procedures. 4.2 In order to use this instrument someone with Radiation Safety Training must be present 5. Setup Procedures 5.1 The liquid nitrogen reservoir must be filled at least an hour before using the XRF

2 5.2 Turn on the computer towers SOP: EDAX Eagle III Microspot XRF Page 2 of The order the computers are turned on matters, so first turn on the silver EDAX tower and then the black PC tower. PC Tower EDAX Tower Cooling Tower Viewing Window X-ray Indicator Chamber Door Figure 2: XRF Towers Figure 1: Eagle III XRF Make sure that both blue lights are visible on the EDAX tower 6. Hardware 6.1 Handles With both handles up, the chamber is pumping and the x-ray source is on With one handle up and one down, chamber is venting and the x-ray source is off With both handles down, the chamber will open once the venting process is complete 6.2 Loading a Sample Turn both handles down and wait for the door to open Place the sample on the stage within the drawn white box If the sample is a powder make sure to pile it up a little so the x-rays do not pass through Close the door and turn both handles up 6.3 Lights and controls Turn on the Power Key Wait for the Pump Indicator AND Vacuum Indicator to turn on Place Samples Here HV Button Vent Indicator Pump Indicator Vacuum Indicator Power Key Figure 3: Chamber Stage Figure 4: Indicator Panel

3 SOP: EDAX Eagle III Microspot XRF Page 3 of 6 7. Viewing the Sample 7.1 Set up With your sample in the chamber and the machine on, open the Vision 32 software icon on the desktop (Fig. 5) 7.2 Driving Make sure the U-Probe Control tab is selected (Fig. 6) Control stage motion with the Joystick Control (Fig. 6) by clicking to place the red crosshairs The red crosshair represents the center of the stage, and the blue crosshair represents the center of the chamber Stage motion may also be controlled using the Stage Control (Fig. 6) scroll bars, or by inputting numbers into the Stage Control boxes Main Toolbar U Probe Control Tab Figure 5: Software Icon Image Window Voltage Current Stage Controls Characterization Peaks Magnification Joystick Control Autofocus Data Bar Figure 6: Vision 32 Software

4 SOP: EDAX Eagle III Microspot XRF Page 4 of Check the stage position by looking into the Viewing Window (Fig. 2) For smaller adjustments, click the Joystick Control Button (Fig. 6), which will enable you to drag the Image Window (Fig. 6) The red circle in the Image Window represents the area to be scanned 7.3 Focusing Focus by adjusting the stage height Adjust the stage height using the Stage Control (Sec ) When the sample is roughly in focus, click the Autofocus button (Fig. 6) If the Autofocus fails to run, this means the focus wasn t close enough With the image now in focus, click the Magnification button (Fig. 6) to toggle to the higher magnification (there are only two magnifications on the XRF) Focus at the higher magnification, adjust position if necessary (Sec ) 8. Collecting a Spectrum 8.1 Getting Counts Press the HV (High Voltage) Button on the Indicator Panel (Fig. 4) to turn on the source The Counts Per Second (CPS) can be found in the Data Bar (Fig. 6), and ideally should be over Beware having too many counts and saturating the detector, if the counts show up red during a scan they will need to be lowered Counts are can be adjusted by changing the voltage or current in the U-Probe tab (Fig. 5) Although the best settings differ by sample, a good place to start would be 30 kv and 750 ua The Dead Time (DT) found in the Data Bar, should be between 20-40% As Dead Time affects the number of counts, 20-40% is the ideal balance between signal and saturation Adjust the Dead Time by changing the Amp Time located on the Main Toolbar (Fig. 6) 8.2 Generating Data Once the CPS is satisfactory, it is now possible to collect data If necessary clear any previous data by clicking the Clear button on the Main Toolbar Click Start on the Main Toolbar to start collecting data The data will collect for a preset amount of time or until the Stop button on the Main Toolbar is clicked 9. Characterizing a Spectrum 9.1 Determining Elements Make sure the Characterization tab is selected

5 SOP: EDAX Eagle III Microspot XRF Page 5 of Click the Auto button within this tab. This will give the computer s best guess of what elements match the peaks WARNING Automatic Characterization is not a foolproof approach. It is not necessarily correct, but only close. The user will need to make adjustments only using the computer s guess as a starting point Select an element in the Sidebar, under the Identified Elements (Fig.7) that was generated by the auto characterization Vertical bars should appear and line up with the peaks of the element selected If the bars and peaks do not match up this is likely an indication the element is not present To delete an selected element that doesn t seem to fit, simply click the Delete button Check to see if another element would fit the peak better using the Z+ and Z- buttons Z+ will step to the next element on the periodic table, while Z- will step to the next element down Follow this procedure with every element that has been identified If a characteristic peak has not been identified it may be necessary to add an element in 9.2 Adding Elements Right click on a peak Possible Elements (Fig. 7) should appear Select the possible elements to judge their validity Remember, to judge the accuracy the vertical bars and peaks should match up Alternatively, simply use the Z+ and Z- buttons (Sec ) to find an element Click Add 9.3 Peak Fit Figure 7: Sidebar To check the accuracy of the elements found, added, and deleted perform a Peak Fit Peak Fit will make an outline of the peaks that should exist based on the elements chosen The peaks that exist and the expected peaks should fit each other well Characterization Tab Identified Elements Possible Elements

6 9.3.4 To run a Peak Fit simply click the Peak Fit button 10. Quantifying Results SOP: EDAX Eagle III Microspot XRF Page 6 of Quantifying gives such useful information as atomic percent and percent by weight With the spectrum characterized (Sec.9) select the Conc menu A table containing the quantification should appear Make sure to save this as a csv. file type 11. Shutting Down 11.1 Saving If saving a quantification table make sure to save it as a csv. file If saving a spectrum, save as a spc. file to edit the data in the future If saving a spectrum, save as a jpg. file to view it on another computer 11.2 Powering Down Go back to the U-probe Control (Fig. 6) Turn down the voltage and current to their lowest settings using the slider bars Turn one handle down (Fig. 2) to begin venting of the chamber and turn off the source Once vented, turn the other handle down to remove samples from the chamber Turn the Power Key (Fig. 2) off Shut chamber door and turn ONE handle up to secure the door (but not pump down) Exit the software Shutdown the PC Turn off the EDAX tower (Fig. 1) 11.3 Process Summary 12. Revision History Rev Date Originator Description of Changes 1 8/09/2010 Kathryn Ecsedy First Draft

SOP: Polyvar Met Light Microscope

SOP: Polyvar Met Light Microscope SOP Polyvar Met Light Microscope Page 1 of 8 SOP: Polyvar Met Light Microscope 1. Scope 1.1 This document describes the standard operating procedure (SOP) for the Polyvar Met Light Microscope. 2. Table

More information

Basic Operating Instructions for Strata Dual Beam 235 FIB/SEM

Basic Operating Instructions for Strata Dual Beam 235 FIB/SEM Basic Operating Instructions for Strata Dual Beam 235 FIB/SEM Warning Always adjust your specimen height before closing the chamber door to make sure your specimen will not hit the bottom of the lens;

More information

Using the Hitachi 3400-N VP-SEM

Using the Hitachi 3400-N VP-SEM Using the Hitachi 3400-N VP-SEM Opening the Chamber to Load Specimens (This may also be done later using the software) 1. Click the AIR button on the front of the machine: 2. Wait a few minutes until you

More information

RENISHAW INVIA RAMAN SPECTROMETER

RENISHAW INVIA RAMAN SPECTROMETER STANDARD OPERATING PROCEDURE: RENISHAW INVIA RAMAN SPECTROMETER Purpose of this Instrument: The Renishaw invia Raman Spectrometer is an instrument used to analyze the Raman scattered light from samples

More information

Title: Amray 1830 SEM#2 Semiconductor & Microsystems Fabrication Laboratory Revision: D Rev Date: 03/18/2016

Title: Amray 1830 SEM#2 Semiconductor & Microsystems Fabrication Laboratory Revision: D Rev Date: 03/18/2016 Approved by: Process Engineer / / / / Equipment Engineer 1 SCOPE The purpose of this document is to detail the use of the Amray 1830 SEM. All users are expected to have read and understood this document.

More information

This procedure assumes the user is already familiar with basic operation of the SEM and the MiraTC interface.

This procedure assumes the user is already familiar with basic operation of the SEM and the MiraTC interface. Tescan MIRA3 SEM: EDS using EDAX TEAM Nicholas G. Rudawski ngr@ufl.edu Cell: (805) 252-4916 Office: (352) 392-3077 Last updated: 12/04/17 This procedure assumes the user is already familiar with basic

More information

JSM 6060 LV SCANNING ELECTRON MICROSCOPE STANDARD OPERATING PROCEDURES

JSM 6060 LV SCANNING ELECTRON MICROSCOPE STANDARD OPERATING PROCEDURES JSM 6060 LV SCANNING ELECTRON MICROSCOPE STANDARD OPERATING PROCEDURES RULES All users must go through a series of standard operation procedure training. For more information contact: Longlong Liao Teaching

More information

Operating Procedures for MICROCT1 Nikon XTH 225 ST

Operating Procedures for MICROCT1 Nikon XTH 225 ST Operating Procedures for MICROCT1 Nikon XTH 225 ST Ensuring System is Ready (go through to ensure all windows and tasks below have been completed either by you or someone else prior to mounting and scanning

More information

Dickinson College Department of Geology

Dickinson College Department of Geology Dickinson College Department of Geology Title: Equipment: BASIC OPERATION OF THE SCANNING ELECTRON MICROSCOPE (SEM) JEOL JSM-5900 SCANNING ELECTRON MICROSCOPE Revision: 2.2 Effective Date: 1/29/2003 Author(s):

More information

ALTURA EDS. Rev. 0915

ALTURA EDS. Rev. 0915 ALTURA EDS Rev. 0915 Enable the Oxford PC Enable the Altura-EDS under Dual Beam Tools in Coral. Or enter your NETID and password directly into the Oxford PC. Warning: Ion-milling, GISs/microprobe, and

More information

Standard Operating Procedure for the Amray 1810 Scanning Electron Microscope Version: 29 NOVEMBER 2014

Standard Operating Procedure for the Amray 1810 Scanning Electron Microscope Version: 29 NOVEMBER 2014 Standard Operating Procedure for the Amray 1810 Scanning Electron Microscope Version: 29 NOVEMBER 2014 1. Utility Requirements a. System power is supplied by two 120 VAC/20 A circuits. When doing maintenance

More information

JEOL 6500 User Manual

JEOL 6500 User Manual LOG IN to your session on the computer to the left of the microscope. Starting Conditions 1. Press Ctrl-Alt-Del and log on to the microscope computer. Click on JEOL PC SEM 6500 icon. Click yes if message

More information

MIF ZEISS LSM510 CONFOCAL USER PROTOCOL

MIF ZEISS LSM510 CONFOCAL USER PROTOCOL MIF ZEISS LSM510 CONFOCAL USER PROTOCOL START-UP Turn on the Mercury Bulb Power Supply (if needed). Power-on the Control Box. Turn on the computer. Open the LSM 510 software. Choose Scan New Images and

More information

Horiba LabRAM ARAMIS Raman Spectrometer Revision /28/2016 Page 1 of 11. Horiba Jobin-Yvon LabRAM Aramis - Raman Spectrometer

Horiba LabRAM ARAMIS Raman Spectrometer Revision /28/2016 Page 1 of 11. Horiba Jobin-Yvon LabRAM Aramis - Raman Spectrometer Page 1 of 11 Horiba Jobin-Yvon LabRAM Aramis - Raman Spectrometer The Aramis Raman system is a software selectable multi-wavelength Raman system with mapping capabilities with a 400mm monochromator and

More information

1. Preliminary sample preparation

1. Preliminary sample preparation FEI Helios NanoLab 600 standard operating procedure Nicholas G. Rudawski ngr@ufl.edu (352) 392 3077 (office) (805) 252-4916 (cell) Last updated: 03/02/18 What this document provides: an overview of basic

More information

RAITH e-line OPERATING INSTRUCTIONS

RAITH e-line OPERATING INSTRUCTIONS RAITH e-line OPERATING INSTRUCTIONS 1) LOADING A SAMPLE a. Start the system i. On the Column PC (Right side monitor [R]), select the SmartSEM icon to on the desktop to begin the column software. ii. On

More information

1.1. Log on to the TUMI system (you cannot proceed further until this is done).

1.1. Log on to the TUMI system (you cannot proceed further until this is done). FEI DB235 SEM mode operation Nicholas G. Rudawski ngr@ufl.edu (805) 252-4916 1. Sample loading 1.1. Log on to the TUMI system (you cannot proceed further until this is done). 1.2. The FIB software (xp)

More information

FE-SEM SU-8020 Operating manual (Preliminary version)

FE-SEM SU-8020 Operating manual (Preliminary version) FE-SEM SU-8020 Operating manual (Preliminary version) 2016/04/11 Seimitsu Bunseki sitsu lab. Starting up 1.Turn on the Display switch. Windows OS is starting up 2. Select the user SU-8000. 3. Click the

More information

TRAINING MANUAL. Olympus FV1000

TRAINING MANUAL. Olympus FV1000 TRAINING MANUAL Olympus FV1000 September 2014 TABLE OF CONTENTS A. Start-Up Procedure... 1 B. Visual Observation under the Microscope... 1 C. Image Acquisition... 4 A brief Overview of the Settings...

More information

Scanning Electron Microscope FEI INSPECT F50. Step by step operation manual

Scanning Electron Microscope FEI INSPECT F50. Step by step operation manual Scanning Electron Microscope FEI INSPECT F50 Step by step operation manual Scanning Electron Microscope, FEI Inspect F50 FE-SEM-F Observation Flow Saving Data And Analysis Specimen preparation Error check

More information

LSM 510 Training Notes

LSM 510 Training Notes LSM 510 Training Notes Turning on the system Turn on the arc lamp, found on the bench top left of the microscope. This supplies light for epifluorescence for viewing your samples through the microscope.

More information

MEASUREMENT CAMERA USER GUIDE

MEASUREMENT CAMERA USER GUIDE How to use your Aven camera s imaging and measurement tools Part 1 of this guide identifies software icons for on-screen functions, camera settings and measurement tools. Part 2 provides step-by-step operating

More information

ARCHICAD Introduction Tutorial

ARCHICAD Introduction Tutorial Starting a New Project ARCHICAD Introduction Tutorial 1. Double-click the Archicad Icon from the desktop 2. Click on the Grey Warning/Information box when it appears on the screen. 3. Click on the Create

More information

Zeiss LSM 510 Confocor III Training Notes. Center for Cell Analysis & Modeling

Zeiss LSM 510 Confocor III Training Notes. Center for Cell Analysis & Modeling Zeiss LSM 510 Confocor III Training Notes Center for Cell Analysis & Modeling Confocor 3 Start Up Go to System Module Turn on Main Switch, System/ PC, and Components Switches Do you need the arc lamp?

More information

SEM OPERATION IN LOW VACUUM MODE

SEM OPERATION IN LOW VACUUM MODE SEM OPERATION IN LOW VACUUM MODE Instructions for JEOL 5800 LV The EVAC light of the SEM specimen chamber should be already lit when you approach the SEM & the SEM will have been left in the high vacuum

More information

LSM 510 Meta Training Notes

LSM 510 Meta Training Notes LSM 510 Meta Training Notes Turning on the system Turn on X-Cite power supply. This supplies light for epifluorescence for viewing your samples through the microscope. Turn on the remote control switch.

More information

Standard Operating Procedure

Standard Operating Procedure CENTER FOR NANOSCALE SCIENCE AND ENGINEERING Standard Operating Procedure Microscope Software Brian Wajdyk Page 1 of 6 Important Images are not to be saved to the computer. They will be deleted without

More information

Bruker Optical Profilometer SOP Revision 2 01/04/16 Page 1 of 13. Bruker Optical Profilometer SOP

Bruker Optical Profilometer SOP Revision 2 01/04/16 Page 1 of 13. Bruker Optical Profilometer SOP Page 1 of 13 Bruker Optical Profilometer SOP The Contour GT-I, is a versatile bench-top optical surface-profiling system that can measure a wide variety of surfaces and samples. Contour GT optical profilers

More information

SEM Training Notebook

SEM Training Notebook SEM Training Notebook Lab Manager: Dr. Perry Cheung MSE Fee-For-Service Facility Materials Science and Engineering University of California, Riverside March 8, 2018 (rev. 3.5) 1 Before you begin Complete

More information

Figure 1 The Raith 150 TWO

Figure 1 The Raith 150 TWO RAITH 150 TWO SOP Figure 1 The Raith 150 TWO LOCATION: Raith 150 TWO room, Lithography area, NanoFab PRIMARY TRAINER: SECONDARY TRAINER: 1. OVERVIEW The Raith 150 TWO is an ultra high resolution, low voltage

More information

SEM Training Notebook

SEM Training Notebook SEM Training Notebook Lab Manager: Dr. Perry Cheung MSE Fee-For-Service Facility Materials Science and Engineering University of California, Riverside December 21, 2017 (rev. 3.4) 1 Before you begin Complete

More information

ZEISS EVO SOP. May 2017 ELECTRON OPTICS

ZEISS EVO SOP. May 2017 ELECTRON OPTICS ZEISS EVO SOP May 2017 ELECTRON OPTICS The patented EVO column is the area of the SEM, where electrons are emitted, accelerated, deflected, focused, and scanned. Main characteristics of the EVO optics

More information

Operating Checklist for using the Scanning Electron. Microscope, JEOL JSM 6400.

Operating Checklist for using the Scanning Electron. Microscope, JEOL JSM 6400. Smith College August 2009 Operating Checklist for using the Scanning Electron Microscope, JEOL JSM 6400. CONTENT, page no. Pre-Check 1 Startup 1 Specimen Insertion 2 Filament Saturation 2 Beam Alignment

More information

Title: Leica SP5 Confocal User Manual

Title: Leica SP5 Confocal User Manual Title: Leica SP5 Confocal User Manual Date of first issue: 23/10/2015 Date of review: Version: Admin For assistance or to report an issue Office: CG07 or 05 Email: Igmm-imaginghelpdesk@igmm.ed.ac.uk Website:

More information

Nikon Eclipse Ti A1-A Confocal Operating Manual. Start-up. Microscope

Nikon Eclipse Ti A1-A Confocal Operating Manual. Start-up. Microscope Nikon Eclipse Ti A1-A Confocal Operating Manual Start-up 1. Turn on Excite Fluorescent light power supply- metal halide. a. Cool down as for mercury bulb b. Wheel closed liquid light guide 2. Turn on power

More information

FEI Quanta 200 ESEM Basic instructions

FEI Quanta 200 ESEM Basic instructions FEI Quanta 200 ESEM Basic instructions Desktop and then start the UI. If the computer has restarted and you need to login, Username: supervisor and Password: supervisor Log-in to the Microscope using the

More information

OPERATION OF THE HITACHI S-450 SCANNING ELECTRON MICROSCOPE. by Doug Bray Department of Biological Sciences University of Lethbridge

OPERATION OF THE HITACHI S-450 SCANNING ELECTRON MICROSCOPE. by Doug Bray Department of Biological Sciences University of Lethbridge OPERATION OF THE HITACHI S-450 SCANNING ELECTRON MICROSCOPE by Doug Bray Department of Biological Sciences University of Lethbridge Revised September, 2000 Note: The terms in bold in this document represent

More information

Picture Style Editor Ver Instruction Manual

Picture Style Editor Ver Instruction Manual ENGLISH Picture Style File Creating Software Picture Style Editor Ver. 1.18 Instruction Manual Content of this Instruction Manual PSE stands for Picture Style Editor. In this manual, the windows used in

More information

JEOL 6700 User Manual 05/18/2009

JEOL 6700 User Manual 05/18/2009 JEOL 6700 User Manual 05/18/2009 LOG IN to your session on the computer to the right of the microscope. Starting Conditions 1. Click the button and read the Penning Gauge to ensure that the microscope

More information

MIF ZEISS VIOLET CONFOCAL ZEN 2009 PROTOCOL

MIF ZEISS VIOLET CONFOCAL ZEN 2009 PROTOCOL MIF ZEISS VIOLET CONFOCAL ZEN 2009 PROTOCOL START-UP On the Switchbox, turn both black switches to the ON position. Wait for the microscope to boot up completely (watch the screen on the side of the microscope).

More information

QUICK-START FOR UNIVERSAL VLS 4.6 LASER! FRESH 21 SEPTEMBER 2017

QUICK-START FOR UNIVERSAL VLS 4.6 LASER! FRESH 21 SEPTEMBER 2017 QUICK-START FOR UNIVERSAL VLS 4.6 LASER! FRESH 21 SEPTEMBER 2017 The laser is quite safe to use, but it is powerful; using it requires your full caution, attention and respect. Some rules of the road:

More information

Nikon AZ100. Laser Scanning Macro Confocal Microscope. Jordan Briscoe Adam Fries Kyle Marchuk Kaitlin Corbin. May 2017.

Nikon AZ100. Laser Scanning Macro Confocal Microscope. Jordan Briscoe Adam Fries Kyle Marchuk Kaitlin Corbin. May 2017. Nikon AZ100 Laser Scanning Macro Confocal Microscope Jordan Briscoe Adam Fries Kyle Marchuk Kaitlin Corbin May 2017 Contents 1 Introduction 2 2 Hardware - Startup 2 3 Software/Operation 4 3.1 Multidimensional

More information

QUICKSTART GUIDE: WIDEFIELD HWF1 Zeiss Cell Observer Live Cell Imaging System (HAMMERSMITH, L BLOCK, ROOM 314) Imperial College London

QUICKSTART GUIDE: WIDEFIELD HWF1 Zeiss Cell Observer Live Cell Imaging System (HAMMERSMITH, L BLOCK, ROOM 314) Imperial College London Imperial College London Facility for Imaging by Light Microscopy QUICKSTART GUIDE: WIDEFIELD HWF1 Zeiss Cell Observer Live Cell Imaging System (HAMMERSMITH, L BLOCK, ROOM 314) Observing Life As It Happens

More information

The operation manual of spotlight 300 IR microscope

The operation manual of spotlight 300 IR microscope The operation manual of spotlight 300 IR microscope Make sure there is no sample under the microscope and then click spotlight on the desktop to open the software. You can do imaging with the image mode

More information

Adobe Photoshop CS2 Workshop

Adobe Photoshop CS2 Workshop COMMUNITY TECHNICAL SUPPORT Adobe Photoshop CS2 Workshop Photoshop CS2 Help For more technical assistance, open Photoshop CS2 and press the F1 key, or go to Help > Photoshop Help. Selection Tools - The

More information

KoPa Scanner. User's Manual A99. Ver 1.0. SHENZHEN OSTEC OPTO-ELECTRONIC TECHNOLOGY CO.,LTD.

KoPa Scanner. User's Manual A99. Ver 1.0. SHENZHEN OSTEC OPTO-ELECTRONIC TECHNOLOGY CO.,LTD. KoPa Scanner A99 User's Manual Ver 1.0 SHENZHEN OSTEC OPTO-ELECTRONIC TECHNOLOGY CO.,LTD. http://www.ostec.com.cn Content Chapter 1 Start... 1 1.1 Safety Warnings and Precautions... 1 1.2 Installation

More information

Tinker Tuesday Project - Fabric Engraving

Tinker Tuesday Project - Fabric Engraving Tinker Tuesday Project - Fabric Engraving 1. Open CorelDRAW and create a new document. On the toolbar on the left side of the screen, select the Basic Shapes tool icon. This will allow you to create simple

More information

The user should already be familiar with operation of the instrument in STEM mode, use of the Microscope Control interface, and TIA.

The user should already be familiar with operation of the instrument in STEM mode, use of the Microscope Control interface, and TIA. FEI Tecnai F20 S/TEM: EDS system operation Nicholas G. Rudawski ngr@ufl.edu (805) 252-4916 (352) 392-3077 Last updated: 01/22/18 The user should already be familiar with operation of the instrument in

More information

Contents STARTUP MICROSCOPE CONTROLS CAMERA CONTROLS SOFTWARE CONTROLS EXPOSURE AND CONTRAST MONOCHROME IMAGE HANDLING

Contents STARTUP MICROSCOPE CONTROLS CAMERA CONTROLS SOFTWARE CONTROLS EXPOSURE AND CONTRAST MONOCHROME IMAGE HANDLING Operations Guide Contents STARTUP MICROSCOPE CONTROLS CAMERA CONTROLS SOFTWARE CONTROLS EXPOSURE AND CONTRAST MONOCHROME IMAGE HANDLING Nikon Eclipse 90i Operations Guide STARTUP Startup Powering Up Fluorescence

More information

Picture Style Editor Ver Instruction Manual

Picture Style Editor Ver Instruction Manual ENGLISH Picture Style File Creating Software Picture Style Editor Ver. 1.12 Instruction Manual Content of this Instruction Manual PSE is used for Picture Style Editor. In this manual, the windows used

More information

Operating the Hitachi 7100 Transmission Electron Microscope Electron Microscopy Core, University of Utah

Operating the Hitachi 7100 Transmission Electron Microscope Electron Microscopy Core, University of Utah Operating the Hitachi 7100 Transmission Electron Microscope Electron Microscopy Core, University of Utah Follow the procedures below when you use the Hitachi 7100 TEM. Starting Session 1. Turn on the cold

More information

R I T. Title: Wyko RST Plus. Semiconductor & Microsystems Fabrication Laboratory Revision: A Rev Date: 05/23/06 1 SCOPE 2 REFERENCE DOCUMENTS

R I T. Title: Wyko RST Plus. Semiconductor & Microsystems Fabrication Laboratory Revision: A Rev Date: 05/23/06 1 SCOPE 2 REFERENCE DOCUMENTS Approved by: Process Engineer / / / / Equipment Engineer 1 SCOPE The purpose of this document is to detail the use of the Wyko RST Plus. All users are expected to have read and understood this document.

More information

g. Click once on the left vertical line of the rectangle.

g. Click once on the left vertical line of the rectangle. This drawing will require you to a model of a truck as a Solidworks Part. Please be sure to read the directions carefully before constructing the truck in Solidworks. Before submitting you will be required

More information

Zeiss 780 Training Notes

Zeiss 780 Training Notes Zeiss 780 Training Notes Turn on Main Switch, System PC and Components Switches 780 Start up sequence Do you need the argon laser (458, 488, 514 nm lines)? Yes Turn on the laser s main power switch and

More information

Standard Operating Procedure

Standard Operating Procedure Standard Operating Procedure Nanosurf Atomic Force Microscopy Operation Facility NCCRD Nanotechnology Center for Collaborative Research and Development Department of Chemistry and Engineering Physics The

More information

BX-61: Brightfield Instruction /Continue to scroll for Fluorescent Instuctions

BX-61: Brightfield Instruction /Continue to scroll for Fluorescent Instuctions BX-61: Brightfield Instruction /Continue to scroll for Fluorescent Instuctions Starting up: Schematic of Olympus BX-61. 1. Turn on Olympus microscope power box (left of microscope) with toggle switch on

More information

House Design Tutorial

House Design Tutorial House Design Tutorial This House Design Tutorial shows you how to get started on a design project. The tutorials that follow continue with the same plan. When you are finished, you will have created a

More information

Zeiss LSM 780 Protocol

Zeiss LSM 780 Protocol Zeiss LSM 780 Protocol 1) System Startup F Please note the sign-up policy. You must inform the facility at least 24 hours beforehand if you can t come; otherwise, you will receive a charge for unused time.

More information

Renishaw InVia Raman microscope

Renishaw InVia Raman microscope Laser Spectroscopy Labs Renishaw InVia Raman microscope Operation instructions 1. Turn On the power switch, system power switch is located towards the back of the system on the right hand side. Wait ~10

More information

Zeiss 880 Training Notes Zen 2.3

Zeiss 880 Training Notes Zen 2.3 Zeiss 880 Training Notes Zen 2.3 1 Turn on the HXP 120V Lamp 2 Turn on Main Power Switch Turn on the Systems PC Switch Turn on the Components Switch. 3 4 5 Turn on the PC and log into your account. Start

More information

PANalytical X pert Pro Gazing Incidence X-ray Reflectivity User Manual (Version: )

PANalytical X pert Pro Gazing Incidence X-ray Reflectivity User Manual (Version: ) University of Minnesota College of Science and Engineering Characterization Facility PANalytical X pert Pro Gazing Incidence X-ray Reflectivity User Manual (Version: 2012.10.17) The following instructions

More information

University of Minnesota Nano Fabrication Center Standard Operating Procedure Equipment Name:

University of Minnesota Nano Fabrication Center Standard Operating Procedure Equipment Name: Equipment Name: Coral Name: Nanoimprinter Revision Number: 1.1 Model: NX-B200 Revisionist: M. Fisher Location: Bay 4 Date: 2/12/2010 1 Description Nanonex NX-B200 nanoimprinter is another method of transfer

More information

Strata DB235 FESEM FIB

Strata DB235 FESEM FIB Strata DB235 FESEM FIB Standard Operating Procedure Revision: 5.0 Last Updated: August 16/2016, revised by Li Yang Overview This document will provide a detailed operation procedure of the Focused Ion

More information

LSM 710 Confocal Microscope Standard Operation Protocol

LSM 710 Confocal Microscope Standard Operation Protocol LSM 710 Confocal Microscope Standard Operation Protocol Basic Operation Turning on the system 1. Switch on Main power switch 2. Switch on System / PC power button 3. Switch on Components power button 4.

More information

Photoshop CC: Essentials

Photoshop CC: Essentials Photoshop CC: Essentials Summary Workspace Overview... 2 Exercise Files... 2 Selection Tools... 3 Select All, Deselect, And Reselect... 3 Adding, Subtracting, and Intersecting... 3 Working with Layers...

More information

Using the AmScope Microscope Cameras

Using the AmScope Microscope Cameras Using the AmScope Microscope Cameras Part 1 Setup. In order to use the camera, you will need: a) the camera system; b) a computer running the camera software. The camera system is contained in a Pelican

More information

Leica SPEII confocal microscope. Short Manual

Leica SPEII confocal microscope. Short Manual Leica SPEII confocal microscope Short Manual Switching ON sequence: 1. Turn on the Workstation under the bench (top, far right). 2. Turn on the Supply Unit - Laser box (big green switch first and then

More information

FEI Tecnai G 2 F20 Operating Procedures

FEI Tecnai G 2 F20 Operating Procedures FEI Tecnai G 2 F20 Operating Procedures 1. Startup (1) Sign-up in the microscope log-sheet. Please ensure you have written an account number for billing. (2) Log in to the computer: Login to your account

More information

Guide to Operating the Bruker FT-IR Microscope I. Basic Spectrum Acquisition with Vertex 70

Guide to Operating the Bruker FT-IR Microscope I. Basic Spectrum Acquisition with Vertex 70 Guide to Operating the Bruker FT-IR Microscope I. Basic Spectrum Acquisition with Vertex 70 Susheng Tan, Ph.D. NanoScale Fabrication and Characterization Facility, University of Pittsburgh SB60 Benedum

More information

Scribble Maps Tutorial

Scribble Maps Tutorial Scribble Maps Tutorial Go to the homepage of Scribble Maps here: h t t p : / / w w w. s c r i b b l e m a p s. c o m / Getting to know the Interface Scribble Maps is a free online mapping application with

More information

Horiba Jobin-Yvon LabRam Raman Confocal Microscope (GERB 120)

Horiba Jobin-Yvon LabRam Raman Confocal Microscope (GERB 120) Horiba Jobin-Yvon LabRam Raman Confocal Microscope (GERB 120) Please contact Dr. Amanda Henkes for training requests and assistance: 979-862-5959, amandahenkes@tamu.edu Hardware LN 2 FTIR FTIR camera 1

More information

Powder diffractometer operation instructions D8 Advance with a Cu Kα sealed tube and Lynxeye MARCH 30, 2016

Powder diffractometer operation instructions D8 Advance with a Cu Kα sealed tube and Lynxeye MARCH 30, 2016 Powder diffractometer operation instructions D8 Advance with a Cu Kα sealed tube and Lynxeye MARCH 30, 2016 1 SAFETY. For radiation safety materials refer to http://www.ehs.wisc.edu/raddevices.htm. There

More information

Model SU3500 Scanning Electron Microscope

Model SU3500 Scanning Electron Microscope Model SU3500 Scanning Electron Microscope Modified and Parts taken from Hitachi Easy Operation Guide. Before using the Model SU3500 SEM, be sure to read the [GENERAL SAFETY GUIDELINES] in the instruction

More information

ThermaViz. Operating Manual. The Innovative Two-Wavelength Imaging Pyrometer

ThermaViz. Operating Manual. The Innovative Two-Wavelength Imaging Pyrometer ThermaViz The Innovative Two-Wavelength Imaging Pyrometer Operating Manual The integration of advanced optical diagnostics and intelligent materials processing for temperature measurement and process control.

More information

Standard Operating Procedure Hitachi UHR CFE SU8230 SEM

Standard Operating Procedure Hitachi UHR CFE SU8230 SEM Standard Operating Procedure Hitachi UHR CFE SU8230 Yale West Campus Materials Characterization Core ywcmatsci.yale.edu ESC II, Room E119F 810 West Campus Drive West Haven, CT 06516 Version 1.1, May 2016

More information

Applications Note. Successfully using the Model 8000 NERO dental x-ray machine timer performance. max to evaluate

Applications Note. Successfully using the Model 8000 NERO dental x-ray machine timer performance. max to evaluate Successfully using the Model 8000 NERO dental x-ray machine timer performance max to evaluate Information in this article applies to Model 8000 NERO max firmware release 2.3 or higher. Introduction The

More information

ScanArray Overview. Principle of Operation. Instrument Components

ScanArray Overview. Principle of Operation. Instrument Components ScanArray Overview The GSI Lumonics ScanArrayÒ Microarray Analysis System is a scanning laser confocal fluorescence microscope that is used to determine the fluorescence intensity of a two-dimensional

More information

LSM 800 Confocal Microscope Standard Operation Protocol

LSM 800 Confocal Microscope Standard Operation Protocol LSM 800 Confocal Microscope Standard Operation Protocol Turning on the system 1. Switch on the Main switch (labeled 1 and 2 ) mounted on the wall. 2. Turn the Laser Key (labeled 3 ) 90 clockwise for power

More information

Check that the pneumatic hose is disconnected!!!! (unless your using the BSE detector, of course)

Check that the pneumatic hose is disconnected!!!! (unless your using the BSE detector, of course) JEOL 7000F BASIC OPERATING INSTRUCTIONS-Ver.-2.0 Note: This is minimal operation checklist and does not replace the other reference manuals. Read the manual for Specimen Exchange (JEOL 7000 Specimen Exchange

More information

LSM 780 Confocal Microscope Standard Operation Protocol

LSM 780 Confocal Microscope Standard Operation Protocol LSM 780 Confocal Microscope Standard Operation Protocol Basic Operation Turning on the system 1. Sign on log sheet according to Actual start time 2. Check Compressed Air supply for the air table 3. Switch

More information

Mastering Your. Embroidery Software V6.0. Owner s Workbook - Part 1

Mastering Your. Embroidery Software V6.0. Owner s Workbook - Part 1 Mastering Your Mastering Your Embroidery Software V6.0 Owner s Workbook - Part 1 1 Table of Contents Introduction... 3 Class 1: Getting Started... Class Overview... 4 Four Bonus programs in BERNINA Embroidery

More information

Microscopy from Carl Zeiss

Microscopy from Carl Zeiss Microscopy from Carl Zeiss Contents Page Contents... 1 Introduction... 1 Starting the System... 2 Introduction to ZEN Efficient Navigation... 5 Setting up the microscope... 10 Configuring the beam path

More information

IBIL setup operation manual for SynerJY software version

IBIL setup operation manual for SynerJY software version IBIL setup operation manual for SynerJY software version 1.8.5.0 Manual version 1.0, 31/10/2008 Author: Carlos Marques Equipment Managers: Carlos Marques, +351219946084, cmarques@itn.pt Luís Alves, +351219946112,

More information

CRL MASS SPECTROMETRY FACILITY INSTRUMENT USER MANUAL MALDI MICRO. Operating Instructions. Basement Spec Lab:

CRL MASS SPECTROMETRY FACILITY INSTRUMENT USER MANUAL MALDI MICRO. Operating Instructions. Basement Spec Lab: CRL MASS SPECTROMETRY FACILITY INSTRUMENT USER MANUAL MALDI MICRO Operating Instructions Basement Spec Lab: 00.097 crs Page of 2 This is a guide to using the MALDI Micro for those who have received training.

More information

JEOL JEM-1400 Transmission Electron Microscope Operating Instructions

JEOL JEM-1400 Transmission Electron Microscope Operating Instructions JEOL JEM-1400 Transmission Electron Microscope Operating Instructions Anti-contamination device Objective aperture Objective aperture translation knobs Specimen holder Pump/air switch Left hand control

More information

Editing and Digitizing in EDS III

Editing and Digitizing in EDS III Editing and Digitizing in EDS III Design Editing Tablet and On-Screen Digitizing Embroidery and Chenille Stitching Scanning Compatibilities Part Number 110221-01, Revision A A Saurer Group Company 1575

More information

Quilt-Pro 6. Creating a Panel Quilt

Quilt-Pro 6. Creating a Panel Quilt QP6 Lessons posted Page 1 Creating a Panel Quilt Quilt-Pro 6 I have seen some really beautiful fabric panels that would look absolutely gorgeous in a quilt. Panel quilts can be made quickly or can be as

More information

WORD ART - CHANGING LETTERING SPACING

WORD ART - CHANGING LETTERING SPACING CHANGING LETTERING SIZE Enter single letters or words and use the icon to rescale the motif. When the Maintaining Proportions (lock) icon is outlined in white, the design will be resized proportionately.

More information

Working With Drawing Views-I

Working With Drawing Views-I Chapter 12 Working With Drawing Views-I Learning Objectives After completing this chapter you will be able to: Generate standard three views. Generate Named Views. Generate Relative Views. Generate Predefined

More information

An Introduction to Lasercut 5.3 Preparing the Artwork

An Introduction to Lasercut 5.3 Preparing the Artwork An Introduction to Lasercut 5.3 Preparing the Artwork Version 0.1, December 8th 2015 Precautions Introduction Importing from.dxf Setting up the layers Checking the Operations Tips, Mistakes and Problems

More information

Picture Style Editor Ver Instruction Manual

Picture Style Editor Ver Instruction Manual ENGLISH Picture Style File Creating Software Picture Style Editor Ver. 1.15 Instruction Manual Content of this Instruction Manual PSE stands for Picture Style Editor. indicates the selection procedure

More information

House Design Tutorial

House Design Tutorial House Design Tutorial This House Design Tutorial shows you how to get started on a design project. The tutorials that follow continue with the same plan. When you are finished, you will have created a

More information

Getting Started. Right click on Lateral Workplane. Left Click on New Sketch

Getting Started. Right click on Lateral Workplane. Left Click on New Sketch Getting Started 1. Open up PTC Pro/Desktop by either double clicking the icon or through the Start button and in Programs. 2. Once Pro/Desktop is open select File > New > Design 3. Close the Pallet window

More information

UCL Depthmap 7: Axial Line Analysis

UCL Depthmap 7: Axial Line Analysis UCL Depthmap 7: Axial Line Analysis Version 7.12.00c Outline This section explains how to import an which has been saved in DXF format. The DXF is just a line drawing, so it must be converted to an axial

More information

Mediasite Desktop Recorder: Recording a Lecture 2017

Mediasite Desktop Recorder: Recording a Lecture 2017 Mediasite Desktop Recorder: Recording a Lecture 2017 This lesson will show you how to record a presentation using the Mediasite Desktop Recorder. In browser go to - https://http://mediasite.umaryland.edu/mediasite/mymediasite

More information

WITec Alpha 300R Quick Operation Summary October 2018

WITec Alpha 300R Quick Operation Summary October 2018 WITec Alpha 300R Quick Operation Summary October 2018 This document is frequently updated if you feel information should be added, please indicate that to the facility manager (currently Philip Carubia,

More information

Microprobe Power User Part 3: X-Ray Maps

Microprobe Power User Part 3: X-Ray Maps Microprobe Power User Part 3: X-Ray Maps Mike Spilde Spring IOM Seminar September 17, 2008 X-Ray Mapping Why use X-ray maps? Characterize the sample at microscopic scales with relatively high sensitivity.

More information

INTRODUCTION TO DATA STUDIO

INTRODUCTION TO DATA STUDIO 1 INTRODUCTION TO DATA STUDIO PART I: FAMILIARIZATION OBJECTIVE To become familiar with the operation of the Passport/Xplorer digital instruments and the DataStudio software. INTRODUCTION We will use the

More information

Chief Architect X3 Training Series. Layers and Layer Sets

Chief Architect X3 Training Series. Layers and Layer Sets Chief Architect X3 Training Series Layers and Layer Sets Save time while creating more detailed plans Why do you need Layers? Setting up Layer Lets Adding items to layers Layers and Layout Pages Layer

More information

QUICK-START FOR UNIVERSAL VLS 4.6 LASER!

QUICK-START FOR UNIVERSAL VLS 4.6 LASER! QUICK-START FOR UNIVERSAL VLS 4.6 LASER! The laser is quite safe to use, but it is powerful; using it requires your full caution, attention and respect. Some rules of the road: Rules of the road If you

More information