Microscope. & Measurements. Do Now

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1 Do Now Microscope & Measurements How many: 1. Centimeters (cm) in 4 meters (m)? m 2. Decimeters (dm) in 5 meters (m)? dm 3. Centimeters (cm) in 4,000 millimeters (mm) cm 4. Millimeters (mm) in 40 centimeters (cm)? mm 5. How do we measure volume? About how big is that? Length of a big step; baseball bat - Width of your finger - Thickness of a fingernail or dime - Microscope: Identify the parts & Functions **On another page Rapid Fire Scenarios 1. An image is too dark while viewing. What should you do? 2. An image is too blurry or not clear. What should you do? 3. The image is small and you want to zoom in to see it closer. What do you do? 4. You are trying to get the slide to stay in place on the stage. What do you do? 5. You go to pick-up the microscope. How do you do it? 6. You are looking using your LOW POWER objective lens and want to use the adjustment knobs. What do you use? 1

2 7. You are looking using the HIGH POWER objective lens and want to use the adjustment knobs. What do you use Why? Compound Microscope Purpose to and non-living specimens not visible or clear to the naked eye. Mainly used. To view smaller objects, the microscope Total magnification the lens (eyepiece) and the lens combine to magnify an image. Magnification What is magnification? magnification means the lens makes the object appear When you magnify an image more, do you see more or less of an image? Your as you increase magnification Field of view - Lenses and total magnification Looking through the microscope you are viewing an object through how many lenses? 1. There is always on a microscope (can t change) 2. But, microscopes usually have lenses o Low power objective (for us either 4x or magnification) 2

3 o High power objective (for us 40x, depending on microscope being used) Total Magnification the To calculate total magnification Since when you look through a microscope you look through... Simply the lens (eyepiece) by the lens Magnification will be shown on the lenses Eyepiece magnification x Objective magnification = Total Magnification Calculating total magnification Eyepiece Power Objective Power Total Magnification 40x 40x 200x Practice problem: Calculate the low-power and the high-power total magnifications of a microscope with an eyepiece magnification of, a low power objective of 20x, and a high-power objective of 40x. o Low power total magnification = o High power total magnification = Microscope Viewing When focusing on a specimen: 1. Place your slide on the stage and secure it under the stage clips 2. Use the and use the adjustment to focus and find the image 3. Once in focus, the image Why? So you can when you increase magnification because your gets 1000x 4. Finally, switch to the objectives and use the to focus! 3

4 Do Now What do you see under the microscope? In general, images appear and What changes do you observe when going from low power to high power? (Use your hand ) Hmmm. What is resolution? Well, what happens if you move the specimen? If you, when you look through the microscope, it will appear to me moving! Remember everything is BACKWARDS!! How do we make the slides that we are going to look at anyway? Preparing a slide to view is called creating a Creating a Wet-Mount 1. Obtain a clean microscope slide and hold by the edges 2. Using forceps, take your specimen and place on the slide 3. Use a dropper to put one drop of water onto the specimen 4. Take cover slip; hold at a 45 to the slide and until the specimen is covered. 4

5 Why? (45 degree angle) To from getting trapped! Sometimes Dye is added to a wet mount Why? To make a 1. Using a dropper, add dye to the that is covering your specimen. 2. Place a small piece of a on the opposite side of the cover slip 3. The added dye or liquid will travel under the cover slip because the water on the opposite side is being absorbed by the towel! Measuring objects under a microscope Measurement used = ( ) How big is a micrometer (micron)? There are in Where does this fit on our staircase of conversions? Convert me! mm 1 mm 6 mm 2.5 mm um 1,200 um 4,000 um 5

6 Looking at a metric ruler under a microscope The smallest lines you can see that are drawn on a metric ruler are You now know there are um in mm. tell me how bug my field of view is (diameter) in the picture. What is your field of view in: Mm: Um: Regents question What is your field of view? 6

7 Measuring objects under a microscope A B C 7

1.When an object is sharply focused and the slide is moved towards you, in which direction does the

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